Robbins G, Petersen C V, Brozović B
Clin Lab Haematol. 1985;7(3):225-30.
The effect of regular platelet apheresis on lymphocyte count was investigated in 142 platelet donors who donated up to 113 times on Haemonetics Model 30 and Fenwal Model CS-3000 cell separators. Lymphocytopenia (lymphocyte count less than 0.9 X 10(9)/l) was found in 21 out of 113 (18.6%) donors who donated solely on the Haemonetics Model 30 compared with 2 out of 100 (2%) whole blood donors and none of 100 plasmapheresis donors (P less than 0.002). Lymphocyte count was inversely related to the total number of platelet donations (r = 0.51, P less than 0.001) and total estimated lymphocyte loss (r = 0.49, P less than 0.001) suggesting a cumulative effect. T4 and T8 lymphocyte subsets were found to be reduced in each of 13 lymphocytopenic platelet donors tested but T4/T8 ratios were similar to those of controls (2.51 +/- 0.79 and 2.33 +/- 0.58 respectively). No adverse effects on platelet donor health were observed but it is suggested that platelet apheresis protocols should be designed to minimize lymphocyte loss.
对142名血小板捐献者进行了研究，这些捐献者使用Haemonetics Model 30和Fenwal Model CS - 3000细胞分离器进行血小板单采，每人最多捐献113次，以调查定期血小板单采对淋巴细胞计数的影响。在仅使用Haemonetics Model 30进行捐献的113名捐献者中，有21名（18.6%）出现淋巴细胞减少（淋巴细胞计数低于0.9×10⁹/L），相比之下，100名全血捐献者中有2名（2%）出现淋巴细胞减少，100名血浆置换捐献者中无人出现淋巴细胞减少（P＜0.002）。淋巴细胞计数与血小板捐献总数呈负相关（r = 0.51，P＜0.001），与估计的淋巴细胞总损失量呈负相关（r = 0.49，P＜0.001），提示存在累积效应。在检测的13名淋巴细胞减少的血小板捐献者中，T4和T8淋巴细胞亚群均减少，但T4/T8比值与对照组相似（分别为2.51±0.79和2.33±0.58）。未观察到对血小板捐献者健康的不良影响，但建议设计血小板单采方案以尽量减少淋巴细胞损失。
