Holland-Winkler Angelia Maleah, Moore Andrew R, Ansley Jenna K, Fritz Noah A, Bederman Ilya
Department of Kinesiology, Augusta University, Augusta, GA 30909, USA.
Department of Genetics and Genome Sciences, School of Medicine, Case Western University, Cleveland, OH 44106, USA.
Muscles. 2023 Sep 27;2(4):327-337. doi: 10.3390/muscles2040025.
Ketone salt (KS) supplementation induces temporary nutritional ketosis to achieve potential exercise performance and health benefits. Racemic KS includes both D/L isomers of β-hydroxybutyrate, yet commercially available measurement devices (i.e., blood meters) only measure the D variant. The aim of this study was to investigate the efficacy of a blood meter to measure serum β-hydroxybutyrate in comparison with gas chromatography-mass spectrometry (GC-MS) before and 30 min after consuming a placebo or racemic KS. In this triple-blinded cross-over study, 16 healthy adults were administered either a placebo or KS drink, and the circulating β-hydroxybutyrate concentration was measured at baseline (PRE) and 30 min following consumption (POST) using a blood ketone meter and by GC-MS. Compared to the placebo, both GC-MS and the blood meter obtained significantly greater β-hydroxybutyrate levels from PRE to POST time-points after consuming KS. Additionally, GC-MS results showed significantly higher levels of β-hydroxybutyrate with both the placebo and KS at PRE and POST time-points, as compared to the blood meter. These results indicate that (1) even in the absence of KS, the blood meter yields significantly lower β-hydroxybutyrate values than GC-MS, and (2) the inability of the blood meter to measure L-β-hydroxybutyrate values POST KS warrants the further development of publicly available ketone measurement apparatuses.
补充酮盐（KS）可诱导暂时性营养性酮症，以实现潜在的运动表现和健康益处。外消旋KS包含β-羟基丁酸的D/L两种异构体，但市售的测量设备（即血糖仪）仅测量D型变体。本研究的目的是在服用安慰剂或外消旋KS之前及之后30分钟，比较血糖仪与气相色谱-质谱联用仪（GC-MS）测量血清β-羟基丁酸的效果。在这项三盲交叉研究中，16名健康成年人分别服用安慰剂或KS饮料，并在基线期（PRE）和服用后30分钟（POST）使用血酮仪和GC-MS测量循环β-羟基丁酸浓度。与安慰剂相比，服用KS后，从PRE到POST时间点，GC-MS和血酮仪测得的β-羟基丁酸水平均显著升高。此外，GC-MS结果显示，在PRE和POST时间点，安慰剂组和KS组的β-羟基丁酸水平均显著高于血酮仪测得的水平。这些结果表明：（1）即使在未补充KS的情况下，血酮仪测得的β-羟基丁酸值也显著低于GC-MS；（2）血酮仪无法测量服用KS后L-β-羟基丁酸的值，这就需要进一步开发公开可用的酮测量仪器。
