McFarlin Brian K, Vingren Jakob L, Hill David W, Bridgeman Elizabeth A
Applied Physiology Laboratory, University of North Texas, Denton, TX 76203, USA.
Department of Biological Sciences, University of North Texas, Denton, TX 76203, USA.
Muscles. 2023 May 6;2(2):204-217. doi: 10.3390/muscles2020015.
Exercise-induced muscle injury and the subsequent release of Damage-Associated Molecular Patterns (DAMP) result in soreness and inflammation. Dietary supplements may accelerate the rate of recovery by supporting resolution of inflammation. The purpose of this study was to determine if methylsulfonylmethane (MSM) supplementation (30 d prior to exercise and during recovery) altered mRNA expression in LPS-exposed blood leukocytes after a bout of downhill running. Exercise consisted of 60 min of downhill running (-15% grade). Blood (baseline, pre-exercise, 4, 24, 48, and 72 h post-exercise) was diluted (1:10) and combined with LPS (20 µg/mL) for 24 h. Total RNA was isolated from leukocytes and analyzed for 574 immune-associated mRNA (Nanostring nCounter; ROSALIND.BIO). Data were expressed as log2 fold change from baseline for each condition (MSM and placebo). Compared to placebo, MSM supplementation was associated with an improved inflammation response (15 mRNA) and viral immune response (2 mRNA). The largest number of changes were found at 4 and 24 h post-exercise. The key finding in the present study is that MSM supplementation can improve inflammation management and the innate immune response after exercise.
运动引起的肌肉损伤以及随后损伤相关分子模式（DAMP）的释放会导致酸痛和炎症。膳食补充剂可能通过支持炎症消退来加快恢复速度。本研究的目的是确定补充甲基磺酰甲烷（MSM）（运动前30天和恢复期间）是否会改变一次下坡跑后暴露于脂多糖（LPS）的血液白细胞中的mRNA表达。运动包括60分钟的下坡跑（坡度为-15%）。采集血液（基线、运动前、运动后4、24、48和72小时），稀释（1:10）后与LPS（20µg/mL）混合24小时。从白细胞中分离总RNA，并分析574种免疫相关mRNA（Nanostring nCounter；ROSALIND.BIO）。数据表示为每种情况（MSM和安慰剂）相对于基线的log2倍数变化。与安慰剂相比，补充MSM与改善炎症反应（15种mRNA）和病毒免疫反应（2种mRNA）相关。运动后4小时和24小时发现的变化最多。本研究的关键发现是，补充MSM可以改善运动后的炎症管理和先天免疫反应。
