Chen Jing, Lei Jing, Wang Ganggang, Qin Huiling, Yang Li
Department of Endocrinology and Metabolism, Shaoxing People's Hospital, Shaoxing, China.
The First Affiliated Hospital, Shaoxing University, Shaoxing, China.
J Endocrinol. 2025 Aug 21;266(2). doi: 10.1530/JOE-25-0032. Print 2025 Aug 1.
Osteoporosis (OP) is a systemic osteopathy characterized by a decrease in bone density and mass. Human bone mesenchymal stem cells (hBMSCs) exhibit multidirectional differentiation potential and play a critical role in bone metabolism. Herein, we investigated the diagnostic potential of miR-127-3p in OP and elucidated its regulatory role in hBMSCs, thereby providing novel insights into the diagnosis and progression prediction of OP. The relative expression of miR-127-3p was measured via RT-qPCR analysis. ROC curve and logistic analysis were applied to identify the diagnostic value of miR-127-3p for OP. The CCK8 assay and flow cytometry were used to assess cell viability and apoptosis. A luciferase reporter assay was performed to assess the interaction between VAMP2 and miR-127-3p. The bone differentiation markers RUNX2, OCN, and OPN were assayed using RT-qPCR and western blotting. We observed that the expression of miR-127-3p was reduced in patients with OP, allowing it to effectively distinguish these patients from healthy individuals. Transfection with miR-127-3p mimic inhibited hBMSC apoptosis, increased cell viability, and increased RUNX2, OCN, and OPN levels. Furthermore, miR-127-3p regulated hBMSCs via targeting VAMP2. Overexpression of VAMP2 reversed the effects of miR-127-3p on apoptosis, cell viability, and bone differentiation. In conclusion, these findings suggest that miR-127-3p may be a potential diagnostic tool for OP. In addition, miR-127-3p promotes hBMSC viability and differentiation through downregulating VAMP2; this decreases OP progression. Our findings will inform new ideas for the diagnosis and developmental prediction of OP.
骨质疏松症（OP）是一种以骨密度和骨量降低为特征的全身性骨病。人骨髓间充质干细胞（hBMSCs）具有多向分化潜能，在骨代谢中起关键作用。在此，我们研究了miR-127-3p在OP中的诊断潜力，并阐明了其在hBMSCs中的调控作用，从而为OP的诊断和病情进展预测提供了新的见解。通过RT-qPCR分析测量miR-127-3p的相对表达。应用ROC曲线和逻辑分析来确定miR-127-3p对OP的诊断价值。采用CCK8法和流式细胞术评估细胞活力和凋亡情况。进行荧光素酶报告基因检测以评估VAMP2与miR-127-3p之间的相互作用。使用RT-qPCR和蛋白质印迹法检测骨分化标志物RUNX2、OCN和OPN。我们观察到OP患者中miR-127-3p的表达降低，这使其能够有效地区分这些患者与健康个体。用miR-127-3p模拟物转染可抑制hBMSC凋亡，提高细胞活力，并增加RUNX2、OCN和OPN水平。此外，miR-127-3p通过靶向VAMP2调控hBMSCs。VAMP2的过表达逆转了miR-127-3p对凋亡、细胞活力和骨分化的影响。总之，这些发现表明miR-127-3p可能是OP的一种潜在诊断工具。此外，miR-127-3p通过下调VAMP2促进hBMSC活力和分化，从而减缓OP的进展。我们的研究结果将为OP的诊断和病情发展预测提供新思路。
