Huang Jiachun, Yang Haolin, Chai Shuang, Lin Yanping, Zhang Zhihai, Huang Hongxing, Wan Lei
The Third Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Guangzhou University of Chinese Medicine, Guangzhou, Guangdong, China.
Front Pharmacol. 2024 Aug 8;15:1451695. doi: 10.3389/fphar.2024.1451695. eCollection 2024.
Osteoporosis is a major health issue. MicroRNAs (miRNAs) play multiple roles in regulating cell growth and development. High-throughput sequencing technology is widely used nowadays.
To screen for and validate miRNAs associated with osteoporosis.
Bone specimens from patients with (n = 3) and without (n = 3) osteoporosis were collected. High-throughput sequencing was used to screen for miRNAs that were then analyzed using volcano maps, Wayne maps, gene ontology (GO) analysis, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Confirmation of the miRNAs was done using qRT-PCR.
The analysis of sequencing showed that there were 12 miRNAs that were down-regulated and five miRNAs that were upregulated in osteoporosis. GO and KEGG identified these miRNAs as being associated with bone metabolism. The qRT-PCR results showed that miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542-3p exhibited a decrease of 2.27-, 3.00-, 3.48-, 2.67-, 2.41-, and 1.98-fold (all < 0.05) in osteoporosis compared to controls. Conversely, miR-486-3p and miR-486-5p demonstrated an increase of 2.17- and 3.89-fold ( < 0.05) (all < 0.05).
This study utilized high-throughput sequencing to detect miRNAs that were expressed differently in individuals with osteoporosis. In osteoporosis, six miRNAs (miR-140-5p, miR-127-3p, miR-199b-5p, miR-181a-5p, miR-181d-5p, and miR-542) were found to have decreased expression, whereas two miRNAs (miR-486-3p and miR-486-5p) were found to have increased expression. The initial manifestation of various miRNAs might serve as predictive indicators and potentially anticipate the progression of osteoporosis.
骨质疏松症是一个重大的健康问题。微小RNA（miRNA）在调节细胞生长和发育中发挥多种作用。如今高通量测序技术被广泛应用。
筛选并验证与骨质疏松症相关的miRNA。
收集骨质疏松症患者（n = 3）和非骨质疏松症患者（n = 3）的骨标本。使用高通量测序筛选miRNA，然后通过火山图、韦恩图、基因本体（GO）分析和京都基因与基因组百科全书（KEGG）分析进行分析。使用qRT-PCR对miRNA进行验证。
测序分析显示，骨质疏松症中有12个miRNA表达下调，5个miRNA表达上调。GO和KEGG分析确定这些miRNA与骨代谢相关。qRT-PCR结果显示，与对照组相比，骨质疏松症患者中miR-140-5p、miR-127-3p、miR-199b-5p、miR-181a-5p、miR-181d-5p和miR-542-3p的表达分别下降了2.27倍、3.00倍、3.48倍、2.67倍、2.41倍和1.98倍（均<0.05）。相反，miR-486-3p和miR-486-5p的表达分别增加了2.17倍和3.89倍（<0.05）（均<0.05）。
本研究利用高通量测序检测骨质疏松症患者中表达差异的miRNA。在骨质疏松症中，发现6个miRNA（miR-140-5p、miR-127-3p、miR-199b-5p、miR-181a-5p、miR-181d-5p和miR-542）表达降低，而2个miRNA（miR-486-3p和miR-486-5p）表达增加。各种miRNA的初始表现可能作为预测指标，并有可能预测骨质疏松症的进展。
