Keller N, Pouliquen Y
Cornea. 1985;4(2):108-17.
The cornea of Elasmobranchs remains transparent and exhibits a remarkable resistance against swelling when deprived of its endothelium. Sutural fibers, which traverse the corneal stroma anteroposteriorly, may be responsible for this property. We studied the structure of the cornea of Scyliorhinus canicula L., a galeiform Elasmobranch, with special emphasis on the presence of a corneal endothelium and on its appearance during development. Observations were made with the aid of several microscopic techniques: light and specular microscopy, transmission and scanning electron microscopy. We found that the posterior corneal surface was always covered by a fibrillar material. A structural counterpart of endothelial-like cells on the posterior cornea or in the anterior chamber was never observed in adult fishes and in embryos of different stages. In contrast, in Torpedo ocellata, a torpediniform Elasmobranch, endothelial cells were seen. The significance of these findings is discussed in context with the possibility of technical artefacts and compared with other studies.
板鳃亚纲动物的角膜保持透明，并且在去除内皮后对肿胀表现出显著的抵抗力。贯穿角膜基质前后的缝线纤维可能是造成这种特性的原因。我们研究了星鲨（Scyliorhinus canicula L.）（一种鳐形板鳃亚纲动物）角膜的结构，特别关注角膜内皮的存在及其在发育过程中的外观。借助多种显微技术进行了观察：光学显微镜和镜面显微镜、透射电子显微镜和扫描电子显微镜。我们发现角膜后表面总是覆盖着一种纤维状物质。在成年鱼类和不同发育阶段的胚胎中，从未观察到后角膜或前房中有类似内皮细胞的结构对应物。相比之下，在电鳐（Torpedo ocellata）（一种电鳐形板鳃亚纲动物）中，可以看到内皮细胞。结合技术假象的可能性对这些发现的意义进行了讨论，并与其他研究进行了比较。
