Wells P A, Hazlett L D
Curr Eye Res. 1985 Nov;4(11):1097-105. doi: 10.3109/02713688509003356.
Aqueous tears were collected by glass capillary tubes at weekly intervals from 6-9 week old, Swiss-Webster mice. Mouse IgA, IgG1, IgG2a and IgM were quantitated in the tears using sandwich type enzyme linked immunosorbent assays (ELISAs). IgE was quantitated using an indirect two-step ELISA. The results established IgA as the predominant antibody in mouse tears at a level of 110 micrograms/ml. This was determined using a mouse IgA standard which contained both polymeric and monomeric IgA. IgG1, IgG2a and IgM were detected in the tears at low levels, 13 ng/ml, 21 ng/ml and 442 ng/ml, respectively. IgE was not detectable in mouse tears with a lower limit of detection equal to 10 ng/ml. These studies establish the relative levels of IgA, IgG1, IgG2a and IgM in the tears of normal adult mice.
从6至9周龄的瑞士韦伯斯特小鼠中，每周使用玻璃毛细管收集一次水样泪液。采用夹心型酶联免疫吸附测定法（ELISA）对泪液中的小鼠IgA、IgG1、IgG2a和IgM进行定量。采用间接两步ELISA对IgE进行定量。结果表明，IgA是小鼠泪液中的主要抗体，水平为110微克/毫升。这是使用包含聚合型和单体型IgA的小鼠IgA标准品测定得出的。在泪液中检测到低水平的IgG1、IgG2a和IgM，分别为13纳克/毫升、21纳克/毫升和442纳克/毫升。在小鼠泪液中未检测到IgE，检测下限为10纳克/毫升。这些研究确定了正常成年小鼠泪液中IgA、IgG1、IgG2a和IgM的相对水平。
