Weissbach H
Curr Top Cell Regul. 1985;26:469-84. doi: 10.1016/b978-0-12-152826-3.50044-9.
A simplified E. coli in vitro system has been developed to study gene expression based on the synthesis of the first di- or tripeptide of the gene product. Plasmids containing bacterial and chloroplast genes have been used as templates in this system. The expression of the E. coli L10 operon, which is under both transcriptional and translational control, has been investigated in some detail using the dipeptide system. A similar system has been developed, using eukaryotic translation components, to measure the expression of eukaryotic mRNA based on dipeptide formation.
已开发出一种简化的大肠杆菌体外系统，用于基于基因产物首个二肽或三肽的合成来研究基因表达。含有细菌和叶绿体基因的质粒已在该系统中用作模板。利用二肽系统已对受转录和翻译双重控制的大肠杆菌L10操纵子的表达进行了较为详细的研究。已开发出一种类似的系统，利用真核生物翻译组件，基于二肽形成来测量真核生物mRNA的表达。
