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1
In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.大肠杆菌RNA聚合酶β亚基首个二肽的体外合成
Proc Natl Acad Sci U S A. 1982 Aug;79(15):4609-12. doi: 10.1073/pnas.79.15.4609.
2
Translational control of the expression of the beta subunit gene of E. coli RNA polymerase.大肠杆菌RNA聚合酶β亚基基因表达的翻译调控
Biochem Biophys Res Commun. 1983 Jun 29;113(3):1018-25. doi: 10.1016/0006-291x(83)91100-2.
3
In vitro stimulation of Escherichia coli RNA polymerase sigma subunit synthesis by NusA protein.NusA蛋白对大肠杆菌RNA聚合酶σ亚基合成的体外刺激作用。
Gene. 1985;33(2):227-34. doi: 10.1016/0378-1119(85)90097-6.
4
In vitro expression of the Escherichia coli nusA-infB operon.大肠杆菌nusA-infB操纵子的体外表达
J Biol Chem. 1987 Jan 5;262(1):152-7.
5
An in vitro system to measure gene expression based on dipeptide synthesis.一种基于二肽合成来测量基因表达的体外系统。
Curr Top Cell Regul. 1985;26:469-84. doi: 10.1016/b978-0-12-152826-3.50044-9.
6
Escherichia coli integration host factor inhibits the NusA stimulation of RNA polymerase sigma subunit synthesis in vitro.大肠杆菌整合宿主因子在体外抑制NusA对RNA聚合酶σ亚基合成的刺激作用。
Arch Biochem Biophys. 1985 Nov 15;243(1):315-9. doi: 10.1016/0003-9861(85)90801-x.
7
Autogenous and post-transcriptional regulation of RNA polymerase synthesis.RNA聚合酶合成的自体及转录后调控。
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8
Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.核糖体蛋白L10合成的翻译控制发生在第一个肽键形成之前。
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9
Formylmethionyl-tRNA alters RNA polymerase specificity.甲酰甲硫氨酰 - tRNA改变RNA聚合酶的特异性。
Proc Natl Acad Sci U S A. 1980 Feb;77(2):870-4. doi: 10.1073/pnas.77.2.870.
10
In vitro regulation of phage lambda cII gene expression by Escherichia coli integration host factor.大肠杆菌整合宿主因子对噬菌体λ cII基因表达的体外调控
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1
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Post-transcriptional regulation of RNA polymerase II levels in Caenorhabditis elegans.秀丽隐杆线虫中RNA聚合酶II水平的转录后调控
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3
Two initiation sites detected in the small s1 species of reovirus mRNA by dipeptide synthesis in vitro.通过体外二肽合成在呼肠孤病毒mRNA的小s1种类中检测到两个起始位点。
Proc Natl Acad Sci U S A. 1984 Feb;81(4):1084-8. doi: 10.1073/pnas.81.4.1084.
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Simplified in vitro system for study of eukaryotic mRNA translation by measuring di- and tripeptide formation.通过测量二肽和三肽形成来研究真核生物mRNA翻译的简化体外系统。
Proc Natl Acad Sci U S A. 1983 Jun;80(11):3223-6. doi: 10.1073/pnas.80.11.3223.
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In vitro expression and characterization of the translation start site of the psbA gene product (QB protein) from higher plants.高等植物光系统II反应中心蛋白D1（psbA基因产物，QB蛋白）翻译起始位点的体外表达与特性分析
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Proc Natl Acad Sci U S A. 1983 May;80(10):2879-83. doi: 10.1073/pnas.80.10.2879.
7
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8
Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors.起始因子IF2基因的序列：异常的蛋白质特征及与延伸因子的同源性
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9
Growth rate-dependent regulation of RNA polymerase synthesis in Escherichia coli.大肠杆菌中RNA聚合酶合成的生长速率依赖性调控。
Mol Gen Genet. 1985;201(3):379-86. doi: 10.1007/BF00331327.
10
Feedback regulation of RNA polymerase subunit synthesis after the conditional overproduction of RNA polymerase in Escherichia coli.大肠杆菌中RNA聚合酶条件性过量表达后RNA聚合酶亚基合成的反馈调节
Mol Gen Genet. 1986 Jul;204(1):17-23. doi: 10.1007/BF00330181.

本文引用的文献

1
In vivo synthesis of a polycistronic messenger RNA for the ribosomal proteins L11, L1, L10 and L7/12 in Escherichia coli.大肠杆菌中核糖体蛋白L11、L1、L10和L7/12的多顺反子信使核糖核酸的体内合成
Mol Gen Genet. 1981;183(2):277-82. doi: 10.1007/BF00270629.
2
Translational control of ribosomal protein L10 synthesis occurs prior to formation of first peptide bond.核糖体蛋白L10合成的翻译控制发生在第一个肽键形成之前。
Proc Natl Acad Sci U S A. 1981 Jul;78(7):4261-4. doi: 10.1073/pnas.78.7.4261.
3
Pausing and termination of transcription within the early region of bacteriophage T7 DNA in vitro.噬菌体T7 DNA体外早期区域转录的暂停与终止
J Biol Chem. 1981 Mar 25;256(6):2777-86.
4
Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.体外大肠杆菌核糖体蛋白L10合成的自体调控
Proc Natl Acad Sci U S A. 1980 May;77(5):2592-5. doi: 10.1073/pnas.77.5.2592.
5
Use of different tRNASer isoacceptor species in vitro to discriminate between the expression of plasmid genes.在体外使用不同的丝氨酸tRNA同工受体种类来区分质粒基因的表达。
Proc Natl Acad Sci U S A. 1982 Mar;79(5):1466-8. doi: 10.1073/pnas.79.5.1466.
6
Negative regulation of beta and beta' synthesis by RNA polymerase.RNA聚合酶对β和β'亚基合成的负调控。
Mol Gen Genet. 1981;183(3):514-7. doi: 10.1007/BF00268773.
7
Bacteriophage lambda vehicle for the direct cloning of Escherichia coli promoter DNA sequences: feedback regulation of the rplJL-rpoBC operon.用于直接克隆大肠杆菌启动子DNA序列的噬菌体λ载体：rplJL-rpoBC操纵子的反馈调节
Proc Natl Acad Sci U S A. 1980 Apr;77(4):2124-8. doi: 10.1073/pnas.77.4.2124.
8
E. coli ribosomal protein L10 inhibits translation of L10 and L7/L12 mRNAs by acting at a single site.大肠杆菌核糖体蛋白L10通过作用于单个位点来抑制L10和L7/L12 mRNA的翻译。
Nature. 1981 Nov 12;294(5837):190-2. doi: 10.1038/294190a0.
9
The primary structure of Escherichia coli RNA polymerase. Nucleotide sequence of the rpoB gene and amino-acid sequence of the beta-subunit.大肠杆菌RNA聚合酶的一级结构。rpoB基因的核苷酸序列和β亚基的氨基酸序列。
Eur J Biochem. 1981 Jun 1;116(3):621-9. doi: 10.1111/j.1432-1033.1981.tb05381.x.
10
Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro.大肠杆菌RNA聚合酶体外合成的自身及转录后调控
Mol Gen Genet. 1980;179(3):489-96. doi: 10.1007/BF00271738.

大肠杆菌RNA聚合酶β亚基首个二肽的体外合成

In vitro synthesis of the first dipeptide of the beta subunit of Escherichia coli RNA polymerase.

作者信息

Peacock S, Cenatiempo Y, Robakis N, Brot N, Weissbach H

出版信息

Proc Natl Acad Sci U S A. 1982 Aug;79(15):4609-12. doi: 10.1073/pnas.79.15.4609.

DOI:10.1073/pnas.79.15.4609
PMID:6289308
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC346724/
Abstract

Plasmids pNF1337 and pNF1341, which contain part of the L10 operon including the RNA polymerase beta-subunit gene, have been used as templates in vitro to investigate expression of the beta-subunit gene. For these studies, the synthesis of the first dipeptide of the beta subunit, fMet-Val, was measured instead of that of the entire protein. By using this dipeptide system, we studied the effects of RNA polymerase holoenzyme and L factor (nus A gene product) on fMET-Val synthesis and compared the relative effects of the primary and secondary promoters in the L10 operon on expression of the beta-subunit gene. The results show that the inhibitory effect of RNA polymerase on beta-subunit synthesis and the stimulatory effect of L factor occur before formation of the first dipeptide bond. In this in vitro system, the secondary promoters account for about 50% of the total fMet-Val synthesized. Although the primary promoter is sensitive to guanosine 5'-diphosphate 3'-diphosphate in vitro, the secondary promoters are not affected by this nucleotide.

摘要

质粒pNF1337和pNF1341含有L10操纵子的一部分，包括RNA聚合酶β亚基基因，已被用作体外模板来研究β亚基基因的表达。在这些研究中，测量的是β亚基第一个二肽fMet-Val的合成，而不是整个蛋白质的合成。通过使用这个二肽系统，我们研究了RNA聚合酶全酶和L因子（nus A基因产物）对fMET-Val合成的影响，并比较了L10操纵子中初级启动子和次级启动子对β亚基基因表达的相对影响。结果表明，RNA聚合酶对β亚基合成的抑制作用和L因子的刺激作用发生在第一个二肽键形成之前。在这个体外系统中，次级启动子占合成的总fMet-Val的约50%。虽然初级启动子在体外对鸟苷5'-二磷酸3'-二磷酸敏感，但次级启动子不受该核苷酸的影响。