Ma Yanyan, Wei Yuli, Guo Hao, Hui Yingfei, Peng Liping, Zhuang Dengke, Niu Wenting, Yan Yu, Hao Yanrui, Yang Wu
Key Laboratory of Eco-Functional Polymer Materials of the Ministry of Education, Key Lab of Bioelectrochemistry and Environmental Analysis of Gansu Province, College of Chemistry and Chemical Engineering, Gansu International Scientific and Technological Cooperation Base of Water-Retention Chemical Functional Materials, Northwest Normal University, Lanzhou, 730070, People's Republic of China.
Provincial Key Laboratory of Gansu Higher Education for City Environmental Pollution Control, School of Chemistry and Chemical Engineering, Lanzhou City University, Lanzhou, 730070, China.
Mikrochim Acta. 2025 Aug 4;192(9):552. doi: 10.1007/s00604-025-07403-7.
It is essential to develop an innovative technology with high sensitivity and real-time detection for the early diagnosis of ovarian cancer. The cross-disciplinary application of multifunctional materials represents a significant trend in contemporary sensing research. In this paper, a ratio-type fluorescence sensing platform RhB/TFBBPY-COF@PBA was constructed from the covalent organic framework (COF) co-functionalized by phenylboric acid and rhodamine B (RhB). Rapid, sensitive, and accurate detection was achieved of ovarian cancer biomarker, sialic acid (SA) with a wide linear range of 0.5-400 μM, and low limit of detection (LOD) of 0.53 μM. Furthermore, a simple and convenient smartphone-assisted colorimetric sensing mode was developed by analyzing changes in fluorescence RGB values, enabling on-site detection of SA without professional instrument with a LOD of 3.66 μM. In addition, the dynamic fluorescence response of the RhB/TFBBPY-COF@PBA composite probe to SA stimulation was still utilized to design optical anti-counterfeiting labels, enabling information encryption in anti-counterfeiting. This research overcomes the limitations associated with the single functionality of traditional sensing materials and establishes a new paradigm for the application of COF materials in the triad of disease diagnosis, on-site detection, and information security. It is significant for the integration and development of precision medicine and smart materials.
开发一种用于卵巢癌早期诊断的具有高灵敏度和实时检测功能的创新技术至关重要。多功能材料的跨学科应用是当代传感研究的一个重要趋势。本文利用苯基硼酸和罗丹明B（RhB）共功能化的共价有机框架（COF）构建了一种比率型荧光传感平台RhB/TFBBPY-COF@PBA。该平台实现了对卵巢癌生物标志物唾液酸（SA）的快速、灵敏且准确的检测，线性范围宽达0.5 - 400 μM，检测限低至0.53 μM。此外，通过分析荧光RGB值的变化，开发了一种简单便捷的智能手机辅助比色传感模式，无需专业仪器即可实现对SA的现场检测，检测限为3.66 μM。另外，RhB/TFBBPY-COF@PBA复合探针对SA刺激的动态荧光响应仍被用于设计光学防伪标签，实现防伪中的信息加密。本研究克服了传统传感材料单一功能的局限性，为COF材料在疾病诊断、现场检测和信息安全三元组中的应用建立了新的范例。这对精准医学和智能材料的融合与发展具有重要意义。
