Jia Hongping, Li Qianyi, Li Zuopeng, Wang Min, Zhang Sanbing, Zhang Zhiqiang
Department of Chemistry, College of Science, China Agricultural University, Yuanmingyuan West Road 2#, Haidian District, Beijing, 100193, China.
Institute of Applied Chemistry, Shanxi Datong University, No. 5 Xingyun Street, Datong, 037009, China.
J Fluoresc. 2024 Aug 14. doi: 10.1007/s10895-024-03899-z.
Ascorbic acid is very important to the metabolic process of the body, but excessive intake can lead to diarrhea, kidney calculi and stomach cramps. However, complicated production procedures and harsh experimental settings limit many detection methods, and a simpler and more accurate measurement method is needed. In this study, a smartphone-assisted ratiometric fluorescence sensor was developed for the portable analysis of ascorbic acid. Leveraging the catalytic properties of MIL-53(Fe) to expedite the conversion of HO into hydroxyl radicals, thereby facilitating the oxidation of o-phenylenediamine and terephthalic acid bridging ligand. The sensor showcased exceptional sensitivity in detecting ascorbic acid within a linear range of 0.3-100 µM, boasting an impressive limit of detection at 0.15 µM. Furthermore, through the utilization of color extraction RGB values captured by smartphones, accurate detection of ascorbic acid was achieved with a detection limit of 0.4 µM. Real fruit samples exhibited robust spiked recovery rates ranging from 91 to 119%, accompanied by relative standard deviations ≤ 4.7%. The MIL-53(Fe) nanozyme-based smartphone-assisted ratiometric fluorescence sensor offers an ascorbic acid fluorescence detection device that is visible, accurate, sensitive, and reasonably priced.
抗坏血酸对人体的代谢过程非常重要，但过量摄入会导致腹泻、肾结石和胃痉挛。然而，复杂的生产程序和苛刻的实验环境限制了许多检测方法，因此需要一种更简单、更准确的测量方法。在本研究中，开发了一种智能手机辅助的比率荧光传感器，用于抗坏血酸的便携式分析。利用MIL-53(Fe)的催化特性加速HO转化为羟基自由基，从而促进邻苯二胺和对苯二甲酸桥连配体的氧化。该传感器在0.3-100 µM的线性范围内检测抗坏血酸时表现出卓越的灵敏度，检测限低至0.15 µM。此外，通过利用智能手机捕获的颜色提取RGB值，实现了对抗坏血酸的准确检测，检测限为0.4 µM。真实水果样品的加标回收率在91%至119%之间，相对标准偏差≤4.7%。基于MIL-53(Fe)纳米酶的智能手机辅助比率荧光传感器提供了一种可见、准确、灵敏且价格合理的抗坏血酸荧光检测装置。
