Pakiet Alicja, Ciosek Michalina, Lange Oliwia, Duzowska Katarzyna, Janczy Agata, Kapusta Małgorzata, Razghonova Yelyzaveta, Ekman Marcin, Abacajew-Chmyłko Anna, Kabata Paweł, Mika Adriana
Department of Environmental Analysis, Faculty of Chemistry, University of Gdansk, Wita Stwosza 63, Gdansk, 80-308, Poland.
Department of Surgical Oncology, Transplant Surgery and General Surgery, Faculty of Medicine, Medical University of Gdansk, Mariana Smoluchowskiego 17, Gdansk, 80-214, Poland.
Cancer Cell Int. 2025 Aug 4;25(1):296. doi: 10.1186/s12935-025-03928-2.
Breast cancer (BC) remains one of the most common cancers with relatively high mortality and is associated with alterations in fatty acid (FA) metabolism. While typical FAs have been extensively studied, there is increasing evidence for a potential role of very long chain fatty acids (VLCFAs) in cancer growth and progression.
This study involved 54 BC patients from whom samples of malignant tumor, normal fibroglandular tissue, and breast adipose tissue were collected. Their FA content was analyzed by gas chromatography-mass spectrometry. The expression of fatty acid elongases (ELOVLs) and FA-transporting proteins was analyzed in the tissues by RT-PCR and immunofluorescence.
Higher levels of saturated and monounsaturated VLCFAs were found in BC tissues compared to normal tissues (p < 0.001) and in patients' blood compared to healthy controls blood (p < 0.001). However, the level of VLCFAs was lower in BC adipose tissue compared to healthy control adipose tissue (p < 0.001). Interestingly, there were no obvious differences in ELOVL1 mRNA or protein levels between normal and cancer tissues.
Elevated levels of VLCFAs in BC tissue are the result of lipid uptake from outside the tumor rather than in situ synthesis.
乳腺癌（BC）仍然是最常见的癌症之一，死亡率相对较高，且与脂肪酸（FA）代谢改变有关。虽然典型脂肪酸已得到广泛研究，但越来越多的证据表明极长链脂肪酸（VLCFA）在癌症生长和进展中可能发挥作用。
本研究纳入了54例乳腺癌患者，收集了他们的恶性肿瘤、正常纤维腺组织和乳腺脂肪组织样本。通过气相色谱-质谱法分析其脂肪酸含量。通过逆转录-聚合酶链反应（RT-PCR）和免疫荧光法分析组织中脂肪酸延长酶（ELOVLs）和脂肪酸转运蛋白的表达。
与正常组织相比，乳腺癌组织中饱和和单不饱和极长链脂肪酸水平更高（p < 0.001），与健康对照者血液相比，患者血液中的极长链脂肪酸水平更高（p < 0.001）。然而，与健康对照者脂肪组织相比，乳腺癌脂肪组织中的极长链脂肪酸水平更低（p < 0.001）。有趣的是，正常组织和癌组织之间ELOVL1 mRNA或蛋白水平没有明显差异。
乳腺癌组织中极长链脂肪酸水平升高是肿瘤从外部摄取脂质的结果，而非原位合成。
Zotero 插件