Goyal Shivani Popli, Chandratre Gauri A, Rawat Anita, Kondepudi Kanthi Kiran, Badgujar Prarabdh C, Saravanan Chakkaravarthi
Department of Inter-disciplinary Sciences, National Institute of Food Technology Entrepreneurship and Management, Sonipat, Haryana, 131028, India.
Department of Veterinary Public Health & Epidemiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, 125001, India.
Curr Microbiol. 2025 Aug 4;82(9):434. doi: 10.1007/s00284-025-04411-x.
Benzo[a]pyrene (B[a]P), a carcinogenic food contaminant, is implicated in colonic abnormalities, including colorectal cancer. This study evaluated the synergistic potential of a novel synbiotic blend of Lactiplantibacillus plantarum MTCC 25433 (9 log CFU/d) and quercetin (25 mg/kg/d) against B[a]P-induced gut dysregulation in Swiss albino mice. Oral B[a]P exposure (50 mg/kg, twice weekly for 28 days) disrupted the intestinal homeostasis, enhancing lipid peroxidation (+ 44.1%), proinflammatory cytokines levels (TNF-α: + 69.6%, IL-6: + 52.9%), inflammatory transcription factors (Nf-κB: 1.92-fold, Foxa2: 2.92-fold) and mucin expression (Muc-2: 2.9-fold), and perturbed gut barrier integrity (Occludin: 0.61-fold, Cldn-4: 0.14-fold). The synbiotic blend exhibited superior efficacy compared to MTCC 25433 or quercetin alone, mitigating MDA (- 32%), TNF-α (- 66%), IL-6 (- 59%), Nf-κB: - 38%, Foxa2: - 51%, Muc-2 (- 57%), Occludin (+ 129%) and Cld-4 (+ 352%) levels and further resulted in enhanced B[a]P fecal excretion (29.5 to 182 μg/g). Two-way ANOVA analysis confirmed synergistic interactions specifically for reductions in adipose tissue B[a]P levels, metabolic enzyme expression (Cyp1b1), gut barrier integrity (Cldn-4), and microbiome modulation, notably Firmicutes, Lachnospiraceae, Clostridium and Ruminococcus, along with enhanced butyrate concentrations. Histopathological analysis confirmed the protective effects of a synbiotic blend against B[a]P-mediated adipocyte disruption and necrosis in the hepatic and intestinal tissues. Principal component analysis also supported its synergistic efficacy in alleviating B[a]P toxicity. Overall, these findings demonstrated the potent anti-inflammatory, antioxidant and gut-modulating potential of the synbiotic blend, suggesting its potential as a promising dietary approach for mitigating the harmful effects of the food contaminant, B[a]P.
苯并[a]芘（B[a]P）是一种致癌性食品污染物，与包括结直肠癌在内的结肠异常有关。本研究评估了植物乳杆菌MTCC 25433（9 log CFU/d）和槲皮素（25 mg/kg/d）的新型合生元混合物对B[a]P诱导的瑞士白化小鼠肠道失调的协同作用潜力。口服B[a]P（50 mg/kg，每周两次，共28天）破坏了肠道稳态，增强了脂质过氧化（+44.1%）、促炎细胞因子水平（TNF-α：+69.6%，IL-6：+52.9%）、炎症转录因子（Nf-κB：1.92倍，Foxa2：2.92倍）和粘蛋白表达（Muc-2：2.9倍），并扰乱了肠道屏障完整性（闭合蛋白：0.61倍，紧密连接蛋白4：0.14倍）。与单独使用MTCC 25433或槲皮素相比，合生元混合物表现出更高的功效，减轻了丙二醛（-32%）、TNF-α（-66%）、IL-6（-59%）、Nf-κB（-38%）、Foxa2（-51%）、Muc-2（-57%）、闭合蛋白（+129%）和紧密连接蛋白4（+352%）的水平，并进一步导致B[a]P粪便排泄增加（29.5至182μg/g）。双向方差分析证实了在降低脂肪组织B[a]P水平、代谢酶表达（Cyp1b1）、肠道屏障完整性（紧密连接蛋白4）和微生物群调节（特别是厚壁菌门、毛螺菌科、梭菌属和瘤胃球菌属）方面存在协同相互作用，同时丁酸盐浓度增加。组织病理学分析证实了合生元混合物对B[a]P介导的肝脏和肠道组织中脂肪细胞破坏和坏死的保护作用。主成分分析也支持其在减轻B[a]P毒性方面的协同功效。总体而言，这些发现证明了合生元混合物具有强大的抗炎、抗氧化和肠道调节潜力，表明其作为一种有前景的饮食方法，可减轻食品污染物B[a]P的有害影响。
