Department of Basic and Applied Sciences, National Institute of Food Technology Entrepreneurship and Management, Sonipat, Haryana 131028, India.
Healthy Gut Research Group, Food and Nutrition Biotechnology Division, National Agri-Food Biotechnology Institute, S.A.S Nagar, Punjab, 140306, India.
Chemosphere. 2024 Feb;349:140891. doi: 10.1016/j.chemosphere.2023.140891. Epub 2023 Dec 13.
Exposure to benzo[a]pyrene (B[a]P), a major global food safety concern, is often associated with increasing incidence of colorectal cancers. This in-vitro study was focused on the identification of potential B[a]P-adsorbing Lactobacillus strains and evaluation of the ameliorative effect of synbiotic combination of selected Lactobacillus sp. and polyphenols (quercetin or resveratrol) against B[a]P-induced intestinal toxicity in Caco-2 cells. Preliminary studies lead to the selection of Lactiplantibacillus plantarum MTCC 25433 strain that showed 86% of B[a]P adsorption in 2 h as compared to L. rhamnosus GG that showed 74% of B[a]P adsorption. B[a]P adsorption by MTCC 25433 was reduced to 9%, 16% and 20% upon pre-treatment with SDS, NaIO and mutanolysin, attributing the involvement of cell wall proteins and polysaccharides in the adsorption. Additionally, peptidoglycan of both strains adsorbed >50% of B[a]P. In-vitro assays revealed that the selected LAB mitigated the B[a]P-induced epithelial cell damage. Among the polyphenols, quercetin, resveratrol and curcumin, varied in their potency to mitigate B[a]P-induced oxidative stress, with curcumin being least effective. Combinations of selected Lactobacillus sp. and polyphenols were more potent in averting B[a]P-induced toxicity via increase in GSH (17-30 %), SOD (50-88 %), catalase (19-45 %), and reduction in IL-8 secretion (14-28 %) and barrier dysfunction. Principal component analysis affirmed the superior potency of combination of L. plantarum MTCC 25433 and quercetin in averting B[a]P-induced toxicity. Overall, this study highlighted a novel promising strategy of synbiotic combination of Lactobacillus sp. and polyphenols (quercetin or resveratrol) in alleviating the B[a]P-induced toxicity in intestinal epithelial cells.
苯并[a]芘（B[a]P）是一种主要的全球食品安全问题，其暴露通常与结直肠癌发病率的增加有关。本体外研究集中于鉴定潜在的 B[a]P 吸附乳酸菌菌株，并评估选定的乳酸菌和多酚（槲皮素或白藜芦醇）组合的共生体对 B[a]P 诱导的 Caco-2 细胞肠道毒性的改善作用。初步研究导致选择植物乳杆菌 MTCC 25433 菌株，该菌株在 2 小时内显示出 86%的 B[a]P 吸附，而鼠李糖乳杆菌 GG 显示出 74%的 B[a]P 吸附。当用 SDS、NaIO 和甘露聚糖酶预处理时，MTCC 25433 对 B[a]P 的吸附减少到 9%、16%和 20%，这归因于细胞壁蛋白和多糖的参与。此外，两种菌株的肽聚糖吸附了超过 50%的 B[a]P。体外试验表明，所选 LAB 减轻了 B[a]P 诱导的上皮细胞损伤。在所研究的多酚中，槲皮素、白藜芦醇和姜黄素在减轻 B[a]P 诱导的氧化应激方面的效力不同，其中姜黄素的效果最差。选定的乳酸菌和多酚的组合通过增加 GSH（17-30%）、SOD（50-88%）、过氧化氢酶（19-45%）和减少 IL-8 分泌（14-28%）和屏障功能障碍，更有效地避免 B[a]P 诱导的毒性。主成分分析证实了植物乳杆菌 MTCC 25433 和槲皮素组合在避免 B[a]P 诱导的毒性方面的卓越效力。总的来说，这项研究强调了乳酸菌和多酚（槲皮素或白藜芦醇）共生体组合作为一种新颖的有前途的策略，用于减轻肠道上皮细胞中 B[a]P 诱导的毒性。
