Zhu Ruirui, Zou Fangfang, Tian Hao, Mo Hongfang, Chen Qilin, Zeng Xinyu, Han Shan, Shi Deshun, Shang Jianghua, Huang Jieping
Guangxi Key Laboratory of Animal Breeding, Disease Control and Prevention, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, 530005, China.
Guangxi Key Laboratory of Buffalo Genetics, Reproduction and Breeding, Buffalo Research Institute, Nanning, Guangxi, 530001, China.
BMC Genomics. 2025 Aug 4;26(1):723. doi: 10.1186/s12864-025-11883-5.
The intramuscular fat content and the level of unsaturated fatty acids influence meat quality and nutritional value. microRNAs can participate in lipid metabolic processes. Understanding how novel miRNAs regulate lipogenesis and fatty acid metabolism is key to enhancing buffalo meat quality and nutritional value.
In this study, miRNA sequencing was conducted on 36 adipose tissues from six anatomical sites in river buffaloes (Murrah). A total of 1,682 miRNAs were identified in 36 adipose tissues, of which 987 were novel and 695 were known miRNAs. Weighted gene co-expression networks analysis (WGCNA) revealed that bta-novel-miR-25336 was highly expressed in sternum subcutaneous adipose tissue (SSAT) and had a high correlation with unsaturated fatty acids (UFA). Functional studies showed that bta-novel-miR-25336 was positively correlated with the proliferation and adipogenic differentiation of both buffalo preadipocytes and intramuscular adipocytes. Additionally, we used bioinformatics analysis and a 3' untranslated region (3'UTR) luciferase reporter assay to validate acyl-CoA dehydrogenase short chain (ACADS) as a target gene of bta-novel-miR-25336.
In summary, bta-novel-miR-25336 may regulate biological processes associated with cell proliferation, lipid differentiation and unsaturated fatty acid synthesis through ACADS. This study provides a theoretical basis for further elucidating the regulation of lipid metabolism by novel miRNAs in farm animals.
肌内脂肪含量和不饱和脂肪酸水平影响肉的品质和营养价值。微小RNA（microRNA）可参与脂质代谢过程。了解新型微小RNA如何调节脂肪生成和脂肪酸代谢是提高水牛肉品质和营养价值的关键。
本研究对河流型水牛（摩拉水牛）六个解剖部位的36个脂肪组织进行了微小RNA测序。在36个脂肪组织中共鉴定出1682个微小RNA，其中987个为新型微小RNA，695个为已知微小RNA。加权基因共表达网络分析（WGCNA）显示，bta-novel-miR-25336在胸骨皮下脂肪组织（SSAT）中高表达，且与不饱和脂肪酸（UFA）高度相关。功能研究表明，bta-novel-miR-25336与水牛前体脂肪细胞和肌内脂肪细胞的增殖及成脂分化呈正相关。此外，我们通过生物信息学分析和3'非翻译区（3'UTR）荧光素酶报告基因检测，验证了酰基辅酶A脱氢酶短链（ACADS）是bta-novel-miR-25336的靶基因。
总之，bta-novel-miR-25336可能通过ACADS调节与细胞增殖、脂质分化和不饱和脂肪酸合成相关的生物学过程。本研究为进一步阐明新型微小RNA对家畜脂质代谢的调控提供了理论依据。
