Li Yannan, Zhang Dan, Tian Tian, Xie Jing, Wang Xiaolin, Deng Wenjun, Hao Ningbo, Li Changzheng
The Postgraduate Training Base of Jinzhou Medical University (Characteristic Medical Center of the PLA Rocket Force), Beijing, China.
Department of Gastroenterology, Daqing Oilfield General Hospital, Heilongjiang, China.
BMC Gastroenterol. 2025 Aug 4;25(1):552. doi: 10.1186/s12876-025-04159-5.
Dysbiosis of the gut microbiota is a significant factor influencing the progression of hepatitis B-related cirrhosis (HBC). Bile acid (BA) metabolism is increasingly recognized as a key participant in the liver-gut microbiota axis.
A total of 46 patients with HBC and 33 healthy adults were enrolled in this study. The HBC patients were divided into a BA-normal group and a BA-high group. Fecal samples were collected from patients under the conditions of their daily diet, and the 16 S rRNA test was performed for each sample.
Compared with that in healthy adults, the alpha diversity of the gut microbiota in HBC patients significantly changed, with a decrease in beneficial microbiota and an increase in opportunistic pathogens. Notably, Bacilli, Enterobacteriales, Streptococcaceae, Veillonella and Lactobacillales were significantly increased in BA-high patients, whereas Clostridia and Clostridiales were significantly decreased. Akkermansiaceae abundance was reduced in the HBC group, and Lactobacillales was markedly enriched in HBC patients, with its abundance proportionally increasing with increasing BA.
These findings provide critical insights for investigating the gut microbiota‒BA crosstalk in HBC, facilitating the discovery of novel biomarkers for disease monitoring and the development of microbiota-targeted therapeutic strategies modulating BA metabolism to intervene in HBC progression.
This study was registered in the Chinese Clinical Trial Registry (ChiCTR2400090990) on October 17, 2024 (retrospectively registered).
肠道微生物群失调是影响乙型肝炎相关肝硬化（HBC）进展的重要因素。胆汁酸（BA）代谢越来越被认为是肝-肠微生物群轴的关键参与者。
本研究共纳入46例HBC患者和33名健康成年人。HBC患者分为BA正常组和BA高水平组。在患者日常饮食条件下采集粪便样本，对每个样本进行16S rRNA检测。
与健康成年人相比，HBC患者肠道微生物群的α多样性显著改变，有益微生物群减少，机会性病原体增加。值得注意的是，BA高水平患者中芽孢杆菌、肠杆菌目、链球菌科、韦荣球菌属和乳杆菌目显著增加，而梭菌纲和梭菌目显著减少。HBC组中阿克曼菌科丰度降低，HBC患者中乳杆菌目显著富集，其丰度随BA升高而呈比例增加。
这些发现为研究HBC中肠道微生物群与BA的相互作用提供了关键见解，有助于发现用于疾病监测的新型生物标志物，并开发以微生物群为靶点的治疗策略来调节BA代谢，以干预HBC的进展。
本研究于2024年10月17日在中国临床试验注册中心（ChiCTR2400090990）注册（回顾性注册）。
