Majidi Sanaz, Rashtbari Samaneh, Jamei Sina, Dehghan Golamreza
Laboratory of Biochemistry and Molecular Biology, Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.
Bioimpacts. 2025 Jul 13;15:30488. doi: 10.34172/bi.30488. eCollection 2025.
Enzyme-mimic nanomaterials, or nanozymes, have received much attention in fundamental and practical research. Laccases are crucial for environmental remediation and biotechnology. Inspired by the structure of the active site and the electron transfer mechanism of laccase, a simple laccase-like platform was designed in this work.
In this work, ovalbumin (OVA) was isolated and purified from hen egg white and conjugated with the transition metal ion (Cu), resulting in a soluble biopolymer (OVA‒Cu complex). Using a colorimetric method based on guaiacol oxidation, the catalytic performance of the complex was assessed, and its laccase‒like activity was verified.
The kinetic parameters ( and values) of the laccase‒mimic complex were calculated to be 0.026 mM and 0.7 μM min, respectively. The prepared complex showed excellent catalytic activity with a similar value to the natural laccase enzyme at the same mass concentration. Analysis of the synthesized system's capacity to decolorize malachite green (MG) showed its strong decolorizing potential. The outcomes demonstrated that MG can be degraded up to 84% in the presence of the OVA‒Cu complex in one hour. The decolorization metabolites were tested for toxicity against and . The results demonstrated that less hazardous metabolites were produced after degrading MG by the OVA‒Cu complex. In addition, a 5:3:1 artificial neural network (ANN) was created to predict the decolorization efficiency (DE (%)) of MG.
Based on these results, it is evident that the OVA‒Cu complex has the potential to take the role of natural laccases in a variety of biosensing, environmental protection, and biotechnology applications.
酶模拟纳米材料，即纳米酶，在基础研究和实际应用中备受关注。漆酶在环境修复和生物技术中至关重要。受漆酶活性位点结构和电子转移机制的启发，本研究设计了一个简单的类漆酶平台。
本研究从鸡蛋清中分离纯化出卵清蛋白（OVA），并与过渡金属离子（Cu）结合，得到一种可溶性生物聚合物（OVA-Cu复合物）。采用基于愈创木酚氧化的比色法评估该复合物的催化性能，并验证其类漆酶活性。
计算得出类漆酶复合物的动力学参数（Km和Vmax值）分别为0.026 mM和0.7 μM min。制备的复合物在相同质量浓度下表现出优异的催化活性，其Vmax值与天然漆酶相似。对合成体系使孔雀石绿（MG）脱色能力的分析表明其具有很强的脱色潜力。结果表明，在OVA-Cu复合物存在下，MG在1小时内可降解达84%。对脱色代谢产物进行了对[具体生物]和[具体生物]的毒性测试。结果表明，OVA-Cu复合物降解MG后产生的代谢产物危害较小。此外，构建了一个5:3:1的人工神经网络（ANN）来预测MG的脱色效率（DE（%））。
基于这些结果，显然OVA-Cu复合物有潜力在各种生物传感、环境保护和生物技术应用中发挥天然漆酶的作用。
