Tian Shuwei, Zhang Xiaoli, Yu Jiayong, Cai Juan, Wei Danni, Li Siqi, Cai Pengfei, Song Wei, Feng Suihan, Shao Mengle, Li Haizhou
Department of Ophthalmology, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.
Shanghai Institute of Immunity and Infection, Chinese Academy of Sciences, Shanghai, China.
Metabol Open. 2025 Jul 24;27:100380. doi: 10.1016/j.metop.2025.100380. eCollection 2025 Sep.
Adipose tissue, a pivotal metabolic regulator, houses diverse stromal cell populations influencing its dynamic functions. Recent omics studies, including transcriptomics and proteomics, have revealed intricate cellular heterogeneity, yet comprehensive metabolic profiling remains limited. Leveraging fluorescence-activated cell sorting (FACS), we isolated PDGFRα+ DPP4+ and PDGFRα+ DPP4- adipose stromal cells (ASCs) from human orbital adipose tissue (OAT). Integrating gene expression analysis, in vitro adipogenesis assays, and quantitative lipidomics, we characterized their functional and metabolic distinctions. DPP4- ASCs exhibited enhanced adipogenic potential and distinct lipidomic profiles, featuring elevated ceramides and triacylglycerols compared to DPP4+ ASCs. Differential gene expression highlighted metabolic and adipogenic gene signatures reflective of their functional roles in adipose tissue remodeling. Our findings underscore the metabolic heterogeneity within OAT stromal fibroblasts, implicating DPP4- ASCs as potent regulators of adipogenesis and metabolic homeostasis. These insights enhance our understanding of adipose tissue plasticity and may inform therapeutic strategies for conditions like thyroid-associated ophthalmopathy.
脂肪组织作为关键的代谢调节因子，包含多种影响其动态功能的基质细胞群。包括转录组学和蛋白质组学在内的近期组学研究揭示了复杂的细胞异质性，但全面的代谢谱分析仍然有限。利用荧光激活细胞分选技术（FACS），我们从人眼眶脂肪组织（OAT）中分离出PDGFRα+ DPP4+和PDGFRα+ DPP4-脂肪基质细胞（ASC）。通过整合基因表达分析、体外脂肪生成测定和定量脂质组学，我们对它们的功能和代谢差异进行了表征。与DPP4+ ASC相比，DPP4- ASC表现出更强的脂肪生成潜力和独特的脂质组学特征，其神经酰胺和三酰甘油含量升高。差异基因表达突出了反映它们在脂肪组织重塑中功能作用的代谢和脂肪生成基因特征。我们的研究结果强调了OAT基质成纤维细胞内的代谢异质性，表明DPP4- ASC是脂肪生成和代谢稳态的有效调节因子。这些见解加深了我们对脂肪组织可塑性的理解，并可能为甲状腺相关眼病等疾病的治疗策略提供参考。
