Hamel Katie, Robinson Jordan, Rogers Emma, Lassiter Haley, Frazier Trivia, Sanchez Cecilia
Obatala Sciences, Inc., New Orleans, LA, USA.
Methods Mol Biol. 2025;2960:183-201. doi: 10.1007/7651_2024_559.
Adipose tissue is recognized not only as an endocrine organ but also as a reservoir for adipose-derived stromal/stem cells (ASCs). ASCs have stimulated the interest of both the scientific and medical communities due to their therapeutic potential and applications in tissue engineering and regenerative medicine. ASCs are leveraged for their multipotency and their paracrine function. ASC behavior is highly variable and donor dependent. Donor age, body mass index, disease status, sex, and ethnicity can lead to differential overall function and quality. The impact of donor age and passage on ASC behavior has been well documented, impacting cell proliferation and differentiation potential and thus must be taken into careful consideration when conducting in vitro studies. Pooling of ASCs from different donors reduces heterogeneity among individual donors and produces ASCs with a consistent differentiation and paracrine profiles, an advantage for studies in biological aging. This chapter provides a detailed overview for studies related to quality control for ASC pools considering biological and chronological aging in ASCs. There are hallmarks of biological aging and specific assays associated with the evaluation of each hallmark. Nevertheless, here we present the assays that provide a standardized characterization and qualification of donor pools for their regenerative potential, considering chronological and biological age of the pool. The assays included in this chapter are considered quality control standards to evaluate cell proliferation, differentiation, colony-forming units, and cellular senescence from different donor age and cell passage cohorts.
脂肪组织不仅被认为是一个内分泌器官，而且还是脂肪来源的基质/干细胞（ASC）的储存库。由于其治疗潜力以及在组织工程和再生医学中的应用，ASC引起了科学界和医学界的关注。ASC因其多能性和旁分泌功能而被利用。ASC的行为高度可变且依赖于供体。供体年龄、体重指数、疾病状态、性别和种族可导致整体功能和质量的差异。供体年龄和传代对ASC行为的影响已有充分记录，会影响细胞增殖和分化潜能，因此在进行体外研究时必须仔细考虑。汇集来自不同供体的ASC可减少个体供体之间的异质性，并产生具有一致分化和旁分泌特征的ASC，这对于生物衰老研究是一个优势。本章详细概述了与考虑ASC生物衰老和自然衰老的ASC库质量控制相关的研究。生物衰老有其特征，并且有与每个特征评估相关的特定检测方法。然而，在这里，我们介绍了一些检测方法，这些方法根据库的自然年龄和生物年龄，为供体库的再生潜力提供标准化的表征和鉴定。本章中包含的检测方法被视为质量控制标准，用于评估来自不同供体年龄和细胞传代组的细胞增殖、分化、集落形成单位和细胞衰老。
