Chen Di, Zhou Xin, Zhang Yan, Chen Jian
Key Laboratory of Food Nutrition and Functional Food of Hainan Province, Engineering Research Center of Utilization of Tropical polysaccharide resources, Ministry of Education; College of Food Science and engineering, Hainan University, Haikou 570228, PR China.
Analysis and Test Center, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Food Chem X. 2025 Jul 24;29:102839. doi: 10.1016/j.fochx.2025.102839. eCollection 2025 Jul.
This study aimed to isolate oligopeptides with anti-allergic properties from cashew nuts. Cashew nut protein was extracted using subcritical water extraction. Subsequently, cashew nut oligopeptides (COPs) were obtained via a two-step enzymatic hydrolysis involving alkaline proteinase and bromelain. Mass spectrometry analysis identified a specific COP with the sequence Ile-Ile-Ala-Ile-Pro-Ala-Gly-Val-Ala-His. This COP was synthesised to investigate its impact on preventing degranulation. Analysis revealed that COP effectively inhibited the release and production of proinflammatory mediators, including tumour necrosis factor-α, interleukin-6, histamine and β-hexosaminidase and influx of extracellular calcium ions. Furthermore, COP significantly suppressed degranulation and inflammatory responses by inhibiting activation of nuclear factor kappa B signalling pathway in allergic mast cells. These findings suggest that COP possesses strong anti-allergic effects by specifically targeting mast cells degranulation. This s highlights COP's therapeutic potential against allergic inflammatory disorders, and its promising role in treating allergic diseases.
本研究旨在从腰果中分离出具有抗过敏特性的寡肽。采用亚临界水萃取法提取腰果蛋白。随后，通过碱性蛋白酶和菠萝蛋白酶两步酶水解获得腰果寡肽（COPs）。质谱分析鉴定出一种特定的COPs，其序列为Ile-Ile-Ala-Ile-Pro-Ala-Gly-Val-Ala-His。合成该COPs以研究其对防止脱颗粒的影响。分析表明，COPs能有效抑制促炎介质的释放和产生，包括肿瘤坏死因子-α、白细胞介素-6、组胺和β-己糖胺酶以及细胞外钙离子内流。此外，COPs通过抑制变应性肥大细胞中核因子κB信号通路的激活，显著抑制脱颗粒和炎症反应。这些发现表明，COPs通过特异性靶向肥大细胞脱颗粒具有强大的抗过敏作用。这突出了COPs在对抗过敏性炎症性疾病方面的治疗潜力，以及其在治疗过敏性疾病中的前景。
