Yu Xiaochuan, Shi Lijuan, Zhang Yating, Wang Huanan, Wang Huali
Chunliu Campus of Dalian Women and Children's Medical Center (Group), Dalian, China.
Dalian Medical University, Dalian, China.
Stem Cell Rev Rep. 2025 Nov;21(8):2667-2670. doi: 10.1007/s12015-025-10950-4. Epub 2025 Aug 5.
Endometrial injury, particularly intrauterine adhesions (Asherman's syndrome), significantly compromises female fertility. Current treatments, including hysteroscopic surgery, intrauterine barriers, and oral estrogen, have limited efficacy, with postoperative pregnancy rates of 22.2-33.3%. Mesenchymal stem cells (MSCs) offer a promising alternative due to their multilineage differentiation and tissue repair capabilities. This study compared the efficacy of MSCs derived from bone marrow, umbilical cord, adipose tissue, and decidua in repairing damaged endometrium.
Proliferation of MSCs was assessed using the CCK8 assay. In vitro differentiation into endometrial epithelial cells was evaluated via immunofluorescence and Western blot. In vivo, a rat model of intrauterine adhesion was used to assess endometrial repair following MSC infusions.
Bone marrow MSCs had the highest proliferation rate, while adipose-derived MSCs had the lowest. Decidual MSCs exhibited superior differentiation into endometrial epithelial cells and the most effective in vivo repair.
Decidual MSCs show superior efficacy in endometrial repair, highlighting their potential for treating intrauterine adhesions. Future studies should focus on clinical validation and mechanistic exploration.
子宫内膜损伤，尤其是宫腔粘连（阿谢曼综合征），会显著损害女性生育能力。目前的治疗方法，包括宫腔镜手术、宫腔屏障和口服雌激素，疗效有限，术后妊娠率为22.2%-33.3%。间充质干细胞（MSCs）因其多向分化和组织修复能力而提供了一种有前景的替代方法。本研究比较了源自骨髓、脐带、脂肪组织和蜕膜的间充质干细胞在修复受损子宫内膜方面的疗效。
使用CCK8法评估间充质干细胞的增殖情况。通过免疫荧光和蛋白质印迹法评估体外向子宫内膜上皮细胞的分化情况。在体内，使用宫腔粘连大鼠模型评估间充质干细胞输注后的子宫内膜修复情况。
骨髓间充质干细胞的增殖率最高，而脂肪来源的间充质干细胞增殖率最低。蜕膜间充质干细胞在向子宫内膜上皮细胞的分化方面表现优异，且在体内修复效果最为显著。
蜕膜间充质干细胞在子宫内膜修复方面显示出优异的疗效，突出了其治疗宫腔粘连的潜力。未来的研究应集中在临床验证和机制探索上。
