Hou Yaxiao, Duan Linxue, Sun Yifan, Hun Xu
Key Laboratory of Optic-electric Sensing and Analytical Chemistry for Life Science, Ministry of Education; Key Laboratory of Analytical Chemistry for Life Science in Universities of Shandong; Shandong Key Laboratory of Biochemical Analysis; State Key Laboratory of Advanced Optical Polymer and Manufacturing Technology; College of Chemistry and Molecular Engineering, Qingdao, 266042, People's Republic of China.
School of Safety Engineering, China University of Labor Relations, Beijing, 100048, People's Republic of China.
Mikrochim Acta. 2025 Aug 5;192(9):557. doi: 10.1007/s00604-025-07407-3.
Dreissena polymorpha, an invasive species in North America, poses a serious threat to the environment and economy. It suffocates native species and clogs infrastructure, disrupting ecosystems. Since this species can also indicate biological pollution in water bodies, early monitoring can enable effective intervention and control measures to mitigate ecological and economic losses. Herein, a strategy was constructed for Dreissena polymorpha DNA detection with enzyme-assisted target recovery coupling DNAzyme-driven double-cycle signal amplification. This detection method utilizes the signal transduction mode of commercial pregnancy test strips. Magnetic beads conjugated with H1 (MB-H1) are hybridized to human chorionic gonadotropin-coupled H2 (hCG-H2). Target binding triggers toehold-mediated strand displacement. The cleavage of the endonuclease releases the target and hCG-H2 and generates DNAzyme. The DNAzyme, activated by Mn, cleaves MB-ssDNA-hCG substrate, releasing abundant free hCG. This converts the target into free hCG, detected visually via commercial pregnancy test strips. The test strip assay showed high sensitivity with a limit of detection of 3.3 fM and showed great potential for monitoring invasive species in a portable and device-free manner, providing practical advantages for ecological management. This use of existing immediate equipment to enable portable detection of non-primary targets demonstrates high potential and advantage.
多形饰贝是北美一种入侵物种，对环境和经济构成严重威胁。它会使本地物种窒息，并堵塞基础设施，扰乱生态系统。由于该物种还能指示水体中的生物污染，早期监测可采取有效的干预和控制措施，以减轻生态和经济损失。在此，构建了一种利用酶辅助靶标回收与脱氧核酶驱动的双循环信号放大技术检测多形饰贝DNA的策略。这种检测方法利用了商用验孕棒的信号转导模式。与H1偶联的磁珠（MB-H1）与人绒毛膜促性腺激素偶联的H2（hCG-H2）杂交。靶标结合触发链置换反应。核酸内切酶的切割释放出靶标和hCG-H2，并产生脱氧核酶。由锰激活的脱氧核酶切割MB-ssDNA-hCG底物，释放出大量游离的hCG。这将靶标转化为游离的hCG，通过商用验孕棒进行可视化检测。试纸条检测显示出高灵敏度，检测限为3.3 fM，并显示出以便携式和无需设备的方式监测入侵物种的巨大潜力，为生态管理提供了实际优势。利用现有的即时设备实现对非主要靶标的便携式检测显示出巨大的潜力和优势。
