Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
Key Laboratory of Green Chemistry & Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China.
Food Chem. 2025 Feb 1;464(Pt 3):141775. doi: 10.1016/j.foodchem.2024.141775. Epub 2024 Oct 24.
Frequently occurred food contamination issues bring about a tremendous global demand for point-of-care testing (POCT) devices. Pregnancy test strip (PTS) retains the highest POCT market share and a low cost of $0.10 per test, but challenged by limited analytical sensitivity and non-autonomous signal transduction for drink contaminant monitoring. Herein, we developed a DNA walking machine-assisted commercial PTS method for on-site bisphenol A (BPA) analysis. DNAzyme was operated as the efficient autonomous catalytic walker chain to drive the DNA machine for the amplified signal output of BPA in drink samples. A detection limit of 16.8 pg/mL was obtained. The POCT strategy constructed in this work has the characteristics of low cost, convenient operation and high analytical sensitivity, represents an attractive option for biosensing and on-site drink safety monitoring.
经常发生的食品污染问题导致全球对即时检测（POCT）设备的需求巨大。妊娠测试条（PTS）保持着最高的 POCT 市场份额，每个测试的成本低至 0.10 美元，但在饮料污染物监测方面，其分析灵敏度有限，信号传导也非自主。在此，我们开发了一种基于 DNA 行走机器辅助的商用 PTS 方法，用于现场双酚 A（BPA）分析。DNA 酶作为高效的自主催化行走链，驱动 DNA 机器对饮料样品中的 BPA 进行放大信号输出。该方法的检测限为 16.8 pg/mL。本工作构建的 POCT 策略具有成本低、操作方便和分析灵敏度高的特点，为生物传感和现场饮料安全监测提供了一种有吸引力的选择。
