Kimijima Manami, Narisawa Naoki, Hama Yoichiro, Nakagawa-Nakamura Tomoyo, Ito Tatsuro
Nihon University Graduate School of Bioresource Sciences, Bioresource Utilization Sciences, Fujisawa, Kanagawa, Japan.
Department of Applied Biochemistry and Food Science, Faculty of Agriculture, Saga University, Saga, Japan.
BMC Res Notes. 2025 Aug 6;18(1):343. doi: 10.1186/s13104-025-07408-8.
Isofloridoside, a galactose-containing heteroside derived from marine red algae, has potential applications as a sweetener because it can activate the sweetness receptors T1R2/T1R3. The purpose of this study was to investigate the effects of isofloridoside on the growth and sucrose-dependent biofilm formation of the cariogenic bacterium Streptococcus mutans, to evaluate its potential as a caries-preventing agent.
The results showed that S. mutans did not grow when isofloridoside was the sole carbon source. Isofloridoside inhibited sucrose-dependent biofilm formation by S. mutans in a concentration-dependent manner, similar to galactose and glucose, but unlike melibiose and galactose-containing disaccharides. Biofilm inhibition induced by isofloridoside was associated with inhibition of glucosyltransferase activity. Isofloridoside exhibited biofilm inhibition comparable to xylitol, an alternative sugar known to inhibit biofilm formation. The differential effects of isofloridoside and melibiose on biofilm formation may result from the structural differences that affect their interactions with S. mutans enzymes. These findings highlight the potential of galactose and its polysaccharides as regulators of S. mutans biofilm formation, and suggest that isofloridoside is a promising alternative sweetener for caries prevention.
异弗罗里糖苷是一种源自海洋红藻的含半乳糖的杂糖苷，因其能激活甜味受体T1R2/T1R3而具有作为甜味剂的潜在应用价值。本研究的目的是调查异弗罗里糖苷对致龋菌变形链球菌生长和蔗糖依赖性生物膜形成的影响，以评估其作为防龋剂的潜力。
结果显示，当异弗罗里糖苷作为唯一碳源时，变形链球菌无法生长。异弗罗里糖苷以浓度依赖性方式抑制变形链球菌的蔗糖依赖性生物膜形成，这与半乳糖和葡萄糖类似，但与蜜二糖和含半乳糖的二糖不同。异弗罗里糖苷诱导的生物膜抑制与葡糖基转移酶活性的抑制有关。异弗罗里糖苷表现出与木糖醇相当的生物膜抑制作用，木糖醇是一种已知能抑制生物膜形成的替代糖。异弗罗里糖苷和蜜二糖对生物膜形成的不同影响可能源于影响它们与变形链球菌酶相互作用的结构差异。这些发现突出了半乳糖及其多糖作为变形链球菌生物膜形成调节剂的潜力，并表明异弗罗里糖苷是一种有前景的防龋替代甜味剂。
