Department of Chemistry, Pukyong National University, Busan 608-737, Republic of Korea.
J Agric Food Chem. 2010 Jan 13;58(1):578-86. doi: 10.1021/jf902811j.
In the exploration of abundant marine biological resources, edible red alga Laurencia undulata led to two bioactive isolates: floridoside (1) and D-isofloridoside (2). For the first time, the antioxidant properties of both derivatives (1 and 2) were characterized via free radical scavenging using the ESR technique, reactive oxygen species (ROS) inhibition, membrane protein oxidation, myeloperoxidase (MPO) inhibition, gene expression levels of glutathione (GSH) and superoxide dismutase (SOD), and protein expression of MMP-2 and MMP-9. The results demonstrate that floridoside and D-isofloridoside possess significant antioxidant capacity and are potential inhibitors of MMP-2 and MMP-9. These results clarified that these components may be responsible for the relative activities of crude extract from this genus, which is used as folk medicine. Furthermore, the structure-activity relationships were also suggested. Both isomers could be effective candidates for applications in food and pharmaceutical fields as natural marine antioxidants.
在探索丰富的海洋生物资源过程中，可食用的红色海藻仙菜（Laurencia undulata）中分离得到了两种具有生物活性的化合物：佛洛里多苷（1）和 D-佛洛里多苷（2）。这两种衍生物（1 和 2）的抗氧化特性首次通过使用 ESR 技术进行自由基清除、抑制活性氧（ROS）、膜蛋白氧化、髓过氧化物酶（MPO）抑制、谷胱甘肽（GSH）和超氧化物歧化酶（SOD）的基因表达水平以及 MMP-2 和 MMP-9 的蛋白表达来进行了表征。结果表明，佛洛里多苷和 D-佛洛里多苷具有显著的抗氧化能力，并且是 MMP-2 和 MMP-9 的潜在抑制剂。这些结果表明，这些成分可能是该属粗提物具有相对活性的原因，该粗提物被用作民间药物。此外，还提出了结构-活性关系。这两个异构体都可能成为食品和制药领域天然海洋抗氧化剂的有效候选物。
