Alugoju Phaniendra, Suwanchaikasem Pipob, Senabunyarith Apinan, Prasad Ankush, Tencomnao Tewin
Natural Products for Neuroprotection and Anti-Ageing Research Unit, Chulalongkorn University, Bangkok, 10330, Thailand.
Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok, 10330, Thailand.
Biogerontology. 2025 Aug 5;26(4):155. doi: 10.1007/s10522-025-10295-y.
Naringin is an antioxidant flavonoid rich in diverse plant species, including citrus plants. While the antioxidant activity of naringin is well documented, there has been limited research on its anti-aging potential. The aim of this study is to investigate the in vivo anti-aging effects of naringin in the budding yeast Saccharomyces cerevisiae as a model. Our findings showed that naringin substantially increased cell viability during the chronological lifespan of wild-type yeast by mitigating oxidative and apoptotic stress markers. However, naringin did not affect the viability of yeast null mutants lacking antioxidant enzymes (sod2Δ, cta1Δ, ctt1Δ, gpx1Δ, gpx2Δ, gsh1Δ; except sod1Δ and tsa1Δ), but slightly increased the viability of only pep4Δ and fis1Δ mutants, not mca1Δ. Gene expression results indicate that naringin altered the expression of genes associated with the TORC1 signaling pathway and other anti-aging genes such as SIR2 and ATG1. The study's findings also demonstrate that naringin could not increase cell viability of yeast null mutants lacking signaling pathway genes (tor1Δ, rim15Δ ras2Δ, and atg1Δ), except sch9Δ mutant during CLS. Metabolomic studies suggest that naringin treatment affects the levels of diverse class of metabolites such as amino acids, nucleotides and related compounds, vitamins, carbohydrates, and lipids in stationary phase yeast. Altogether, these findings suggest that naringin might exerts its anti-aging effects via modulating the nutrient sensing TORC1 signaling pathway, paving the way for future research to explore other aging associated gene targets.
柚皮苷是一种抗氧化类黄酮，广泛存在于包括柑橘类植物在内的多种植物中。虽然柚皮苷的抗氧化活性已有充分记载，但其抗衰老潜力的研究却很有限。本研究旨在以出芽酵母酿酒酵母为模型，研究柚皮苷在体内的抗衰老作用。我们的研究结果表明，柚皮苷通过减轻氧化应激和凋亡应激标志物，显著提高了野生型酵母在时序寿命期间的细胞活力。然而，柚皮苷对缺乏抗氧化酶的酵母缺失突变体（sod2Δ、cta1Δ、ctt1Δ、gpx1Δ、gpx2Δ、gsh1Δ；除sod1Δ和tsa1Δ外）的活力没有影响，但仅略微提高了pep4Δ和fis1Δ突变体的活力，对mca1Δ突变体则没有影响。基因表达结果表明，柚皮苷改变了与TORC1信号通路以及其他抗衰老基因（如SIR2和ATG1）相关的基因表达。研究结果还表明，柚皮苷在CLS期间不能提高缺乏信号通路基因（tor1Δ、rim15Δ、ras2Δ和atg1Δ）的酵母缺失突变体的细胞活力，但对sch9Δ突变体除外。代谢组学研究表明，柚皮苷处理会影响静止期酵母中多种代谢物的水平，如氨基酸、核苷酸及相关化合物、维生素、碳水化合物和脂质。总之，这些发现表明柚皮苷可能通过调节营养感应TORC1信号通路发挥其抗衰老作用，为未来探索其他衰老相关基因靶点的研究铺平了道路。
