Comparative Analysis of Gut Microbiota from Rats Induced by Se Deficiency and T-2 Toxin.

The aim of this study was to analyze the differences in gut microbiota between selenium deficiency and T-2 toxin intervention rats. Knee joint and fecal samples of rats were collected. The pathological characteristics of knee cartilage were observed by safranin O/fast green staining. DNA was extracted from fecal samples for PCR amplification, and 16S rDNA sequencing was performed to compare the gut microbiota of rats. At the phylum level, (81.39% vs. 77.06%) and (11.11% vs. 14.85%) were dominant in the Se-deficient (SD) group and T-2 exposure (T-2) groups. At the genus level, the relative abundance of (12.62%) and (10.31%) in the SD group were higher. In the T-2 group, the relative abundance of (11.71%) and (9.26%) were higher. At the species level, the high-quality bacteria in the SD group was , and in the T-2 group. and were the most significant biomarkers in the T-2 group. This study analyzed the different compositions of gut microbiota in rats induced by selenium deficiency and T-2 toxin, and revealed the changes in gut microbiota, so as to provide a certain basis for promoting the study of the pathogenesis of Kashin-Beck disease (KBD).