Department of Occupational and Environmental Health, School of Public Health, Xi'an Jiaotong University Health Science Center, Xi'an 710061, China.
MED-X Institute, Center for Immunological and Metabolic Diseases, The First Affiliated Hospital of Xi'an Jiaotong University, Xi'an 710061, China.
Nutrients. 2023 Dec 7;15(24):5027. doi: 10.3390/nu15245027.
The aim of this study was to analyze the differences in gut microbiota between selenium deficiency and T-2 toxin intervention rats. Knee joint and fecal samples of rats were collected. The pathological characteristics of knee cartilage were observed by safranin O/fast green staining. DNA was extracted from fecal samples for PCR amplification, and 16S rDNA sequencing was performed to compare the gut microbiota of rats. At the phylum level, (81.39% vs. 77.06%) and (11.11% vs. 14.85%) were dominant in the Se-deficient (SD) group and T-2 exposure (T-2) groups. At the genus level, the relative abundance of (12.62%) and (10.31%) in the SD group were higher. In the T-2 group, the relative abundance of (11.71%) and (9.26%) were higher. At the species level, the high-quality bacteria in the SD group was , and in the T-2 group. and were the most significant biomarkers in the T-2 group. This study analyzed the different compositions of gut microbiota in rats induced by selenium deficiency and T-2 toxin, and revealed the changes in gut microbiota, so as to provide a certain basis for promoting the study of the pathogenesis of Kashin-Beck disease (KBD).
本研究旨在分析硒缺乏和 T-2 毒素干预大鼠肠道微生物群的差异。收集大鼠的膝关节和粪便样本。通过番红 O/固绿染色观察膝关节软骨的病理特征。从粪便样本中提取 DNA 进行 PCR 扩增，并进行 16S rDNA 测序，以比较大鼠的肠道微生物群。在门水平上，（81.39%比 77.06%）和（11.11%比 14.85%）在硒缺乏（SD）组和 T-2 暴露（T-2）组中占优势。在属水平上，SD 组中 （12.62%）和 （10.31%）的相对丰度较高。在 T-2 组中，（11.71%）和 （9.26%）的相对丰度较高。在种水平上，SD 组的优质细菌为 ，而 T-2 组的优质细菌为 。 和 是 T-2 组中最显著的生物标志物。本研究分析了硒缺乏和 T-2 毒素诱导的大鼠肠道微生物群的不同组成，揭示了肠道微生物群的变化，为促进大骨节病（KBD）发病机制的研究提供了一定依据。
