M Vasuki, Jacob Eunice Swarna, Sivagamasundari P, Kumar P B Praveen
Department of Microbiology, Thanjavur Medical College, Thanjavur, IND.
Cureus. 2025 Jul 5;17(7):e87359. doi: 10.7759/cureus.87359. eCollection 2025 Jul.
One of the leading causes of morbidity worldwide is urinary tract infections (UTIs). We are already running out of antimicrobial drugs in this era of antimicrobial resistance. It is important to know the antimicrobial resistance pattern of all organisms that commonly cause human diseases. The purpose of this investigation was to identify the pathogen that causes UTIs and to characterize the molecular makeup of the isolate of that is resistant to fosfomycin in urine samples. A total of 1104 samples were examined during the June-August 2024 retrospective investigation at our institution. However, only 91 isolates of were detected using standard microbiological methods. The Kirby-Bauer disk diffusion method was used to assess their susceptibility to antimicrobials. Of the 91 recovered , 97% (n = 88) exhibited phenotypic susceptibility to fosfomycin, while the remaining 3 fosfomycin-resistant isolates underwent real-time polymerase chain reaction (RT-PCR). However, the fosA3 gene was detected in only one fosfomycin-resistant isolate. While urinary tract infections are a common and widespread ailment that affects a large number of people worldwide, antibiotics are currently limited in their ability to treat them. Hence, fosfomycin may be the preferred choice of antibiotic.
全球发病的主要原因之一是尿路感染（UTIs）。在这个抗菌药物耐药的时代，我们的抗菌药物已经快用完了。了解所有常见的引起人类疾病的生物体的抗菌药物耐药模式很重要。本研究的目的是确定引起尿路感染的病原体，并对尿液样本中对磷霉素耐药的分离株的分子组成进行表征。在我们机构于2024年6月至8月进行的回顾性调查中，共检查了1104份样本。然而，使用标准微生物学方法仅检测到91株分离株。采用 Kirby-Bauer 纸片扩散法评估它们对抗菌药物的敏感性。在回收的91株分离株中，97%（n = 88）对磷霉素表现出表型敏感性，而其余3株对磷霉素耐药的分离株进行了实时聚合酶链反应（RT-PCR）。然而，仅在一株对磷霉素耐药的分离株中检测到fosA3基因。虽然尿路感染是一种常见且广泛的疾病，影响着全球大量人群，但目前抗生素治疗尿路感染的能力有限。因此，磷霉素可能是抗生素的首选。
