Hemm R D, Arslanoglou L, Pollock J J
Teratology. 1977 Jun;15(3):243-8. doi: 10.1002/tera.1420150305.
Following food restriction of pregnant CD-1 mice, the maternal serum corticosteroid levels and incidence of skeletal and visceral abnormalities of the progeny were determined. Dams from the groups receiving restricted food on gestation days 6 to 15 had increased resorptions, and the progeny had delayed ossification of the skeleton with cleft palate accompanied by delayed ossification of the presphenoid bone. The incidence and intensity of these changes was correlated with the level of food restriction. The incidence of cleft palate for progeny from dams receiving 4.0 to 2.5 g of feed per day ranged from 6.3% to 100.0%, respectively. Serum corticosteroid levels of pregnant control and food restricted dams were similar on gestation day 10, but the levels of food restricted dams were 6 to 10 times greater than controls on gestation day 15. Thus cleft palate, delayed skeletal ossification, and increased resorptions are predictably produced by food restriction of CD-1 mice and were highly correlated with the elevated endogenous serum corticosteroid levels.
对怀孕的CD-1小鼠进行食物限制后，测定了母体血清皮质类固醇水平以及子代骨骼和内脏异常的发生率。在妊娠第6至15天接受食物限制的组中的母鼠有更多的吸收现象，并且子代骨骼骨化延迟，伴有腭裂，同时蝶骨前部骨化也延迟。这些变化的发生率和严重程度与食物限制水平相关。每天接受4.0至2.5克饲料的母鼠所产后代的腭裂发生率分别为6.3%至100.0%。妊娠第10天时，怀孕对照组和食物受限母鼠的血清皮质类固醇水平相似，但在妊娠第15天时，食物受限母鼠的水平比对照组高6至10倍。因此，对CD-1小鼠进行食物限制可预测地导致腭裂、骨骼骨化延迟和吸收增加，并且与内源性血清皮质类固醇水平升高高度相关。
