Mattson J C, Borgerding P J, Craft D L
Stain Technol. 1977 May;52(3):151-8. doi: 10.3109/10520297709116767.
A double fixation method of preparing platelet suspensions for both scanning and transmission electron microscopy is outlined. Prefixation in 0.1% glutaraldehyde allows for immediate preservation of morphologic characteristics induced by experimental procedures, but does not completely destroy platelet surface stickiness. Preservation of surface stickiness allows subsequent production of a platelet pellet for processing for transmission electron microscopy. This pelleting cannot be achieved when higher initial concentrations of glutaraldehyde are used for prefixation. Prefixation in 0.1% glutaraldehyde is also an appropriate initial step for preservation of platelets in suspension for scanning electron microscopy.
本文概述了一种用于扫描电子显微镜和透射电子显微镜的制备血小板悬液的双重固定方法。用0.1%戊二醛进行预固定可立即保存实验操作诱导的形态学特征，但不会完全消除血小板表面的黏性。保留表面黏性可使后续制备用于透射电子显微镜处理的血小板沉淀。当使用更高初始浓度的戊二醛进行预固定时，无法实现这种沉淀。用0.1%戊二醛进行预固定也是将悬浮液中的血小板保存用于扫描电子显微镜的合适初始步骤。
