Heynen M J, Tricot G, Verwilghen R L
Histochemistry. 1984;80(1):79-84. doi: 10.1007/BF00492775.
We have tried to improve existing methods for demonstration of platelet peroxidase (PPO) in human platelets and megakaryocytes by introducing a fixation of 0.1% glutaraldehyde prior to incubation in the DAB medium. This prefixation with low concentration of glutaraldehyde preserves excellent morphological detail and does not inhibit PPO activity. All 23 platelet-rich plasma samples show PPO reaction product in the dense tubular system after incubation in DAB medium with 0.003% H2O2. When 0.01% H2O2 is used in excessive DAB medium, PPO activity can also be demonstrated in platelets and megakaryocytes of bone-marrow cell suspensions. This method can be used for the identification of megakaryoblasts in acute non-lymphocytic leukemia, myelodysplastic syndromes and in blastic crisis of chronic myeloid leukemia. PPO cytochemistry can be combined with postfixation in a OsO4-ruthenium red mixture. This method reveals alpha-granules, dense bodies, microtubuli, glycogen, mitochondria, dense tubular system and invaginated membrane system in the same platelet and is useful for investigation of platelet ultrastructure.
我们试图通过在二氨基联苯胺（DAB）培养基中孵育前引入0.1%戊二醛固定，来改进现有的在人血小板和巨核细胞中显示血小板过氧化物酶（PPO）的方法。这种低浓度戊二醛的预固定能保留极佳的形态细节，且不抑制PPO活性。所有23份富含血小板的血浆样本在含有0.003%过氧化氢（H2O2）的DAB培养基中孵育后，在致密管状系统中均显示出PPO反应产物。当在过量的DAB培养基中使用0.01% H2O2时，在骨髓细胞悬液的血小板和巨核细胞中也能显示PPO活性。该方法可用于急性非淋巴细胞白血病、骨髓增生异常综合征及慢性髓性白血病急变期原巨核细胞的鉴定。PPO细胞化学可与锇酸-钌红混合物后固定相结合。该方法能在同一血小板中显示α颗粒、致密体、微管、糖原、线粒体、致密管状系统和内陷膜系统，有助于血小板超微结构的研究。
