Miles M A, Toye P J, Oswald S C, Godfrey D G
Trans R Soc Trop Med Hyg. 1977;71(3):217-25. doi: 10.1016/0035-9203(77)90012-8.
Culture forms of 17 Trypanosoma cruzi stocks, primarily isolated from a rural area of endemic Chagas disease at São Felipe, Bahia, Brazil, were compared by the electrophoretic patterns of six enzymes: aspartate aminotransferase, alanine aminotransferase, glucose-6-phosphate dehydrogenase, malate dehydrogenase (decarb-oxylating) (NADP+), glucosephosphate isomerase and phosphoglucomutase. Two markedly distinct combinations of isoenzyme patterns were seen, justifying the arrangement of the 17 stocks into two strain-groups, each of which was enzymically homogeneous. One combination was characteristic of the 11 domestic stocks of T. cruzi derived from both human infections and domiciliated animals; the second was characteristic of the six sylvatic stocks derived from opossums and a sylvatic triatomine species. The enzyme patterns were independent of the original host and the type of culture medium used. Distinction of the two strain-groups accords with epidemiological evidence that the domestic and sylvatic transmission cycles in São Felipe do not overlap. It is suggested that the diverse enzyme characters of the two strain-groups circulating in São Felipe reflect diverse origins; the domestic form of T. cruzi probably invaded the area from the south of Brazil with the domestic triatomine vector, Panstrongylus megistus.
对主要从巴西巴伊亚州圣费利佩恰加斯病流行农村地区分离出的17株克氏锥虫的培养形式，通过六种酶的电泳图谱进行了比较：天冬氨酸转氨酶、丙氨酸转氨酶、葡萄糖-6-磷酸脱氢酶、苹果酸脱氢酶（脱羧）（NADP+）、葡萄糖磷酸异构酶和磷酸葡萄糖变位酶。观察到两种明显不同的同工酶模式组合，这使得可以将17株菌株分为两个菌株组，每个菌株组在酶学上都是同质的。一种组合是来自人类感染和家养动物的11株克氏锥虫家养菌株的特征；第二种是来自负鼠和一种野生锥蝽物种的6株野生菌株的特征。酶模式与原始宿主和所用培养基类型无关。两个菌株组的区分与流行病学证据一致，即圣费利佩的家养和野生传播周期不重叠。有人认为，在圣费利佩传播的两个菌株组的不同酶特征反映了不同的起源；克氏锥虫的家养形式可能是随着家养锥蝽媒介大锥蝽从巴西南部侵入该地区的。
