Allen Joanne L, Bushell Rhys N, Noormohammadi Amir H, Whiteley Pam, Ballard Susan A, Valcanis Mary, Browning Glenn F, Marenda Marc S
Asia-Pacific Centre for Animal Health, Melbourne Veterinary School, Faculty of Science, University of Melbourne, Parkville, Victoria, Australia.
Melbourne Veterinary School, Faculty of Science, University of Melbourne, Werribee, Victoria, Australia.
PLoS One. 2025 Aug 6;20(8):e0329807. doi: 10.1371/journal.pone.0329807. eCollection 2025.
The zoonotic bacterium Pasteurella multocida infects a wide range of animals worldwide. While the genetic diversity of this pathogen is well described in production animals, it remains underexplored in companion animals. In Australia, most P. multocida genomes come from commercial poultry. Here, 59 P. multocida clinical isolates obtained from Australian pets, (cats, dogs, rabbits), farm animals (birds, ruminants, porcine) and captive wildlife (Quolls, Serval) between 2006 and 2023 were sequenced and compared to 523 representative RefSeq genomes. Clustering and phylogenomic analyses placed 24/25 Australian pet isolates in a long-branched clade containing several MLST profiles (ST36, ST37, ST171, ST359, ST451 and ST527) also found in human isolates. Genotypes associated with Australian production animals (e.g., ST8, ST9 and ST20 from poultry, ST79 and ST394 from cattle) fell in the main branch of the tree. Minimum spanning tree and SNP analyses suggested several occurrences of cross-species transmission. Mobile genetic elements were found across the P. multocida population, without clustering into any specific phylogenetic, host, or geographic group. However, a 1.8 kb cryptic plasmid (Acc. U51470), previously described in Pasteurella canis strains from South Korea, was detected in 99/289 (34.3%) Australian P. multocida isolates from various hosts, while being largely absent from the rest of the world. Antimicrobial resistance was not detected in the isolates from Australian companion animals and captive wildlife. However, resistances to tetracyclines in bovine isolates (2/10) and ampicillin in avian (1/17) isolates were identified. This study greatly expands our insights on the diversity of P. multocida genomes from Australian companion animals and provides the basis for wider investigations on the molecular epidemiology and diversity of this pathogen, with potential applications to better understand the zoonotic risks associated with this pathogen.
人畜共患病细菌多杀巴斯德菌感染全球范围内的多种动物。虽然这种病原体在生产动物中的遗传多样性已有充分描述，但在伴侣动物中仍未得到充分研究。在澳大利亚，大多数多杀巴斯德菌基因组来自商业家禽。在此，对2006年至2023年间从澳大利亚宠物（猫、狗、兔子）、农场动物（鸟类、反刍动物、猪）和圈养野生动物（袋鼬、薮猫）中获得的59株多杀巴斯德菌临床分离株进行了测序，并与523个代表性的RefSeq基因组进行了比较。聚类和系统发育基因组分析将24/25株澳大利亚宠物分离株置于一个长分支进化枝中，该进化枝包含几个也在人类分离株中发现的多位点序列分型谱（ST36、ST37、ST171、ST359、ST451和ST527）。与澳大利亚生产动物相关的基因型（例如，来自家禽的ST8、ST9和ST20，来自牛的ST79和ST394）位于树的主要分支中。最小生成树和单核苷酸多态性分析表明存在多次跨物种传播。在多杀巴斯德菌群体中发现了移动遗传元件，但未聚集成任何特定的系统发育、宿主或地理组。然而，在来自不同宿主的99/289（34.3%）澳大利亚多杀巴斯德菌分离株中检测到一种1.8 kb的隐蔽质粒（登录号U51470），该质粒先前在来自韩国的犬巴斯德菌菌株中有所描述，而在世界其他地区基本不存在。在澳大利亚伴侣动物和圈养野生动物的分离株中未检测到抗菌药物耐药性。然而，在牛分离株（2/10）中鉴定出对四环素的耐药性，在禽类分离株（1/17）中鉴定出对氨苄西林的耐药性。这项研究极大地扩展了我们对来自澳大利亚伴侣动物的多杀巴斯德菌基因组多样性的认识，并为更广泛地研究该病原体的分子流行病学和多样性提供了基础，有可能应用于更好地了解与该病原体相关的人畜共患病风险。
