[ WRKY12 mediates bolting and flowering by interacting with the and promoters].

Flowering and bolting are important agronomic traits in cruciferous crops such as . Timely flowering can ensure the crop organ yield and quality, as well as seed propagation. The WRKY family plays an important role in regulating plant bolting and flowering, while the function and mechanism of in . remain unknown. To explore its function and mechanism in bolting and flowering of . , we cloned and characterized the gene in . and found that its expression levels were significantly higher in flowers and inflorescences than in leaves. BjuWRKY12 belonged to the Ⅱc subfamily of the WRKY family, and subcellular localization indicated that the protein was located in the nucleus. Ectopic overexpression of in transgenic lines promoted bolting and flowering, leading to significant increases in the expression levels of flowering integrators and . Furthermore, yeast one-hybrid and dual luciferase reporter system assays confirmed that BjuWRKY12 directly bound to the promoters of and , undergoing protein-DNA interactions. This discovery gives new insights into the regulation network and molecular mechanisms of , laying a theoretical foundation for the breeding of high-yield and high-quality varieties of . .