Maswanna Thanaporn, Maneeruttanarungroj Cherdsak
Scientific Instruments Center, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Department of Biology, School of Science, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
World J Microbiol Biotechnol. 2025 Aug 7;41(8):301. doi: 10.1007/s11274-025-04523-3.
Limnophila aromatica, a traditional medicinal plant, has been previously reported to possess notable antimicrobial properties. However, the specific bioactive constituents responsible for this activity remain largely unidentified. This study aimed to isolate, identify, and evaluate the antibacterial potential of compounds from a 100% ethanolic extract of L. aromatica. The crude ethanolic extract exhibited the highest antibacterial activity against Methicillin-resistant Staphylococcus aureus (MRSA) and was subsequently subjected to Diaion HP-20 column chromatography, followed by preparative HPLC. Seven major peaks were identified using UV-Vis spectra, LC-QTOF-MS, and NMR analyses. The antibacterial efficacy of these isolated compounds was assessed using disc diffusion and broth microdilution assays against Bacillus subtilis, S. aureus, MRSA, Escherichia coli, and Pseudomonas aeruginosa. The isolated compounds were identified as norethindrone acetate, isothymusin, nevadensin, gardenin B, 5,3'-dihydroxy-7,8,2'-trimethoxyisoflavone, jasmolin II, and oleanolic acid. Nevadensin, the predominant compound (79.55%), demonstrated potent bactericidal activity against B. subtilis, S. aureus, and MRSA. Jasmolin II and gardenin B also exhibited promising antibacterial effects. Although the disc diffusion assay was limited by compound diffusion, the broth microdilution method confirmed significant MIC values, ranging from 0.59 to 2.86 mg/ml while the MBC values ranged from 0.59 to 12.72 mg/ml. This study highlights nevadensin and other flavonoids from L. aromatica as potential candidates for the development of alternative antibacterial therapies, particularly against drug-resistant Gram-positive bacteria.
香泽兰，一种传统药用植物，此前已有报道称其具有显著的抗菌特性。然而，负责此活性的具体生物活性成分在很大程度上仍未明确。本研究旨在从香泽兰100%乙醇提取物中分离、鉴定并评估化合物的抗菌潜力。粗乙醇提取物对耐甲氧西林金黄色葡萄球菌（MRSA）表现出最高的抗菌活性，随后进行Diaion HP - 20柱色谱，接着进行制备型高效液相色谱。使用紫外 - 可见光谱、液相色谱 - 四极杆飞行时间质谱和核磁共振分析鉴定出七个主要峰。使用纸片扩散法和肉汤微量稀释法针对枯草芽孢杆菌、金黄色葡萄球菌、MRSA、大肠杆菌和铜绿假单胞菌评估这些分离化合物的抗菌效果。分离出的化合物被鉴定为醋酸炔诺酮、异胸腺素、内华达辛、栀子黄素B、5,3'-二羟基 - 7,8,2'-三甲氧基异黄酮、茉莉素II和齐墩果酸。内华达辛是主要化合物（79.55%），对枯草芽孢杆菌、金黄色葡萄球菌和MRSA表现出强效杀菌活性。茉莉素II和栀子黄素B也表现出有前景的抗菌作用。尽管纸片扩散法受化合物扩散限制，但肉汤微量稀释法证实了显著的最低抑菌浓度（MIC）值，范围为0.59至2.86毫克/毫升，而最低杀菌浓度（MBC）值范围为0.59至12.72毫克/毫升。本研究强调香泽兰中的内华达辛和其他黄酮类化合物作为开发替代抗菌疗法的潜在候选物，特别是针对耐药革兰氏阳性菌。
