Kirkik Duygu, Hacimustafaoğlu Fatih, Özkanca Cemre, Kalkanli Taş Sevgi, Elmastaş Mahfuz
Department of Immunology, Hamidiye Medicine Faculty, University of Health Sciences, Istanbul, Türkiye.
Department of Medical Biology, Hamidiye Medicine Faculty, University of Health Sciences, Istanbul, Türkiye.
Sci Rep. 2025 Aug 6;15(1):28673. doi: 10.1038/s41598-025-12550-1.
Crocodile oil has been traditionally used for its medicinal properties, including wound healing and antimicrobial effects. However, scientific validation of its antimicrobial activity remains limited. Although its potential to induce microbial resistance and its safety profile have been proposed in previous literature, these aspects were not addressed experimentally in the current study. This study aimed solely to evaluate the in vitro antimicrobial activity of crocodile oil using dip solution and standard microbiological approaches. Standard bacterial and fungal strains, including Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans, were tested using disk diffusion and minimum inhibitory concentration (MIC) methods. Fatty acid composition was analyzed using GC-FID. The results demonstrated significant antimicrobial activity against Staphylococcus aureus ATCC 29213, Staphylococcus aureus ATCC 43300 (methicillin-resistant), Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans. No inhibition was observed against Staphylococcus epidermidis ATCC 12228 and Klebsiella pneumoniae ATCC 4352, as confirmed by both disk diffusion and MIC methods. MIC values ranged from 187.5 to 500 µL/Ml. The lowest MIC was observed for Escherichia coli (187.5 µL/mL), while MICs of 375 µL/mL were recorded for Staphylococcus aureus ATCC 43300, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans. The highest MIC (500 µL/mL) was observed for Staphylococcus aureus ATCC 29213. While the results are promising, further studies are necessary to evaluate safety, resistance development potential, and in vivo efficacy.
鳄鱼肉一直因其药用特性而被传统使用，包括伤口愈合和抗菌作用。然而，其抗菌活性的科学验证仍然有限。尽管先前的文献中提出了其诱导微生物耐药性的可能性及其安全性，但本研究并未通过实验解决这些问题。本研究仅旨在使用浸液和标准微生物学方法评估鳄鱼肉的体外抗菌活性。使用纸片扩散法和最低抑菌浓度（MIC）方法对包括金黄色葡萄球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌在内的标准细菌和真菌菌株进行了测试。使用气相色谱 - 火焰离子化检测法（GC - FID）分析脂肪酸组成。结果表明，鳄鱼肉对金黄色葡萄球菌ATCC 29213、金黄色葡萄球菌ATCC 43300（耐甲氧西林）、粪肠球菌、大肠杆菌、铜绿假单胞菌和白色念珠菌具有显著的抗菌活性。纸片扩散法和MIC方法均证实，对表皮葡萄球菌ATCC 12228和肺炎克雷伯菌ATCC 4352没有抑制作用。MIC值范围为187.5至500μL/Ml。大肠杆菌的MIC最低（187.5μL/mL），而金黄色葡萄球菌ATCC 43300；粪肠球菌、铜绿假单胞菌和白色念珠菌的MIC为375μL/mL。金黄色葡萄球菌ATCC 29213的MIC最高（500μL/mL）。虽然结果很有前景，但仍需要进一步研究来评估安全性、耐药性发展潜力和体内疗效。
