Liu Hui, Guan Yuan, Xu Like, Hu Ji
Medical College, Suzhou University, SuZhou, Jiangsu, China.
Affiliated First People's Hospital of Endocrinology, Yangzhou University, Yangzhou, Jiangsu, China.
Front Endocrinol (Lausanne). 2025 Jul 23;16:1532560. doi: 10.3389/fendo.2025.1532560. eCollection 2025.
This study investigated the effectiveness of a high-fat diet (HFD) in inducing type 2 diabetes in Sprague-Dawley (SD) rats and evaluated the combined therapeutic effects of Yupingfeng and metformin on glycemic index and intestinal histology. A total of 60 SD rats, equally divided by sex, were randomly allocated to six groups. Body weight was measured weekly, and food intake was recorded. During the initial phase of model induction, blood glucose levels increased significantly, exceeding 17.26 mmol/L by week 8, which was significantly higher than that of the control group (< 0.01). The experimental group received treatment for a total of 7 weeks. Beginning in the third week, the combined administration of Yupingfeng and metformin significantly reduced fasting insulin levels, blood glucose concentrations, and insulin resistance compared to the control group, with further marked reductions observed by the seventh week (< 0.01). Lipid metabolism indicators, including triglycerides (TG), total cholesterol (TCHO), high-density lipoprotein cholesterol (HDL-c), and low-density lipoprotein cholesterol (LDL-c), showed significant reductions in the treatment groups (< 0.01). Liver and kidney function assessments showed that the combination therapy exhibited superior safety compared to single-drug treatments. In addition, hematoxylin and eosin (H&E) staining revealed that HFD-induced diabetes caused inflammatory changes in the duodenum and colon. However, the combination therapy markedly alleviated inflammatory symptoms. Overall, the combined treatment significantly improved blood glucose levels, biochemical parameters, and intestinal tissue integrity.
本研究调查了高脂饮食（HFD）诱导斯普拉格-道利（SD）大鼠患2型糖尿病的有效性，并评估了玉屏风和二甲双胍对血糖指数和肠道组织学的联合治疗效果。总共60只SD大鼠按性别平均分为六组。每周测量体重，并记录食物摄入量。在模型诱导的初始阶段，血糖水平显著升高，到第8周超过17.26 mmol/L，显著高于对照组（<0.01）。实验组共接受7周治疗。从第三周开始，与对照组相比，玉屏风和二甲双胍联合给药显著降低了空腹胰岛素水平、血糖浓度和胰岛素抵抗，到第七周观察到进一步显著降低（<0.01）。包括甘油三酯（TG）、总胆固醇（TCHO）、高密度脂蛋白胆固醇（HDL-c）和低密度脂蛋白胆固醇（LDL-c）在内的脂质代谢指标在治疗组中显著降低（<0.01）。肝肾功能评估表明，联合治疗比单药治疗具有更高的安全性。此外，苏木精-伊红（H&E）染色显示，高脂饮食诱导的糖尿病导致十二指肠和结肠发生炎症变化。然而，联合治疗显著减轻了炎症症状。总体而言，联合治疗显著改善了血糖水平、生化参数和肠道组织完整性。
