Lu Hong-Yu, Shi An-Ning, Wang Jun, Liu Li-Juan, Tang Jiang-Yi, Wang Ya-Jun, Zhu Xue-Zhi, Zhang De-Feng, Liu Li-Yan, Wang Qing
Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Key Laboratory of Fishery Drug Development, Ministry of Agriculture and Rural Areas, Guangdong Provincial, Key Laboratory of Aquatic Animal Immune Technology, Guangzhou 510380, China.
Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang Normal University, Neijiang 641100, China.
Aquac Nutr. 2025 Jul 30;2025:3661772. doi: 10.1155/anu/3661772. eCollection 2025.
Glucoside is the main antinutritional factor (ANF) of rapeseed meal. In the present study, a bacterium with high efficiency in degrading rapeseed glucoside was screened from pond water. The initial concentration of 1.0 × 10 CFU/mL bacterial solution was added to rapeseed meal and fermented for 48 h with a degradation rate of glucoside and isothiocyanate of 67% and 54%, respectively. BSY82 was identified by PacBio third-generation sequencing, and its biology was analyzed. Results showed that BSY82 belongs to . The total length of genome was 4,242,094 bp, among which 89% was coding genes (3,777,298 bp). No virulence factors were predicted, based on a comparative analysis of the strain's coding genes against known virulence factor databases. Functional analysis showed that BSY82 had the ability to produce myrosinase. The activity of myrosinase in the crude enzyme solution of the strain was 1.67 and 2.12 μmol/min by spectrophotometry and high-performance liquid chromatography (HPLC). The results indicated that BSY82 strain could be used as the optimal material in rapeseed meal fermentation detoxification process.
硫代葡萄糖苷是菜籽粕的主要抗营养因子。在本研究中，从池塘水中筛选出一株高效降解菜籽硫代葡萄糖苷的细菌。将初始浓度为1.0×10 CFU/mL的菌液加入到菜籽粕中，发酵48小时，硫代葡萄糖苷和异硫氰酸酯的降解率分别为67%和54%。通过PacBio第三代测序对BSY82进行鉴定，并分析其生物学特性。结果表明，BSY82属于 。基因组全长4,242,094 bp，其中89%为编码基因（3,777,298 bp）。基于该菌株编码基因与已知毒力因子数据库的比较分析，未预测到毒力因子。功能分析表明，BSY82具有产生黑芥子酶的能力。通过分光光度法和高效液相色谱法（HPLC）测定，该菌株粗酶液中黑芥子酶的活性分别为1.67和2.12 μmol/min。结果表明，BSY82菌株可作为菜籽粕发酵脱毒过程中的最佳材料。
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