Li Chongyang, Wang Junli, Guo Fanxi, Zhang Fengyichi, Chen Baochang, Wang Zihan, Cao Di, Yu Zugong
Laboratory of Veterinary Pharmacology and Toxicology, Division of Basic Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.
Front Microbiol. 2025 Jul 23;16:1533892. doi: 10.3389/fmicb.2025.1533892. eCollection 2025.
is a major pathogen that causes acute clinical mastitis and its recurrent episodes in dairy cows.
In this study, a peristaltic pump one-compartment open model was established to investigate the relationship between the pharmacokinetic and pharmacodynamic (PK/PD) indices of cefquinome (CFQ) against . Bactericidal effects of single high-dosage versus multiple low-dosage administrations within the same drug dosage and best-fit dosage were assessed.
Static time-killing curves showed that the population of was not changed when the drug concentration was below 1 × MIC. The maximum antibacterial effect was observed at 24 h, when the concentration exceeded 2 × MIC, showing a reduction by 5.73 log (CFU/mL), and the maximum kill rate was 0.22 h. were cleared at 120 h when the concentration was ≥1 mg/L within single high-dosage groups, except for the 0.28 and 0.5 mg/L groups. The multiple-dose groups decreased below 2.22 log (CFU/mL) at 48 h and increased to 9 log (CFU/mL) at 120 h, but the group of 0.25 mg/L (4, q24) increased at 144 h. As the frequency of administration increased, the lag time increased following a population decline. The correlation coefficients between AUC/MBC, %T > MBC, and the antibacterial effects were 0.90 and 0.99%, respectively. %T > MBC was the best-fit PK/PD parameter of CFQ against . The MIC of S1-S5 strains ranged from 0.0156-0.0625 μg/mL, and biofilm formation ability increased.
In conclusion, CFQ showed good efficacy and time-dependence. This study provides a reference for optimizing CFQ administration in .
是导致奶牛急性临床乳腺炎及其反复发作的主要病原体。
在本研究中，建立了蠕动泵一室开放模型，以研究头孢喹肟（CFQ）对的药代动力学和药效学（PK/PD）指标之间的关系。评估了在相同药物剂量和最佳拟合剂量下单次高剂量与多次低剂量给药的杀菌效果。
静态时间杀灭曲线表明，当药物浓度低于1×MIC时，的数量没有变化。当浓度超过2×MIC时，在24小时观察到最大抗菌效果，显示减少5.73 log（CFU/mL），最大杀灭率为0.22小时。在单次高剂量组中，当浓度≥1 mg/L时，在120小时清除，0.28和0.5 mg/L组除外。多剂量组在48小时降至2.22 log（CFU/mL）以下，在120小时升至9 log（CFU/mL），但0.25 mg/L（4，q24）组在144小时增加。随着给药频率增加，群体下降后的滞后时间增加。AUC/MBC、%T>MBC与抗菌效果之间的相关系数分别为0.90和0.99%。%T>MBC是CFQ对的最佳拟合PK/PD参数。S1-S5菌株的MIC范围为0.0156-0.0625μg/mL，生物膜形成能力增加。
总之，CFQ显示出良好的疗效和时间依赖性。本研究为优化CFQ在中的给药提供了参考。
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