Cholik Rafsan Syabani, Skrypnik Katarzyna, Waśkiewicz Agnieszka, Karaźniewicz-Łada Marta, Suliburska Joanna
Department of Human Nutrition and Dietetics, Poznan University of Life Sciences, Poznań, Poland.
Department of Chemistry, Poznan University of Life Sciences, Poznań, Poland.
Biol Trace Elem Res. 2025 Aug 7. doi: 10.1007/s12011-025-04772-1.
Low-dose aspirin prophylaxis is recommended for women at high risk of preeclampsia. It has been suggested that dietary salicylates may have a similar effect. Despite the known anti-inflammatory properties of salicylates, their influence on trace elements in preeclampsia remains unclear. This research investigated the effect of dietary salicylates and aspirin on iron, zinc, and copper status in rats with NG-nitro-L-arginine methyl ester (L-NAME)-induced preeclampsia. The study involved pregnant Sprague Dawley rats divided into six groups: control group (CH), preeclamptic rats (CP), preeclamptic rats with a low dose of dietary salicylate (LSP), preeclamptic rats with a high dose of dietary salicylate, preeclamptic rats with a low dose of aspirin (LAP), and preeclamptic rats with a high dose of aspirin. The content of trace elements in diets, liver, kidney, heart, spleen, pancreas, femur, brain, and hair was measured using flame atomic absorption spectrometry. Salicylate concentrations in diets, serum, and urine were analyzed using HPLC and UHPLC-MS/MS systems. Administration of L-NAME resulted in elevated blood pressure across groups, and only the LAP group had blood pressure levels comparable to the CH group. Preeclampsia significantly decreased serum hepcidin levels, while salicylates abolished this effect. Salicylate administration significantly decreased iron levels in hair and increased maternal zinc concentrations in the brain. Dietary salicylates markedly increased zinc levels in the placenta. In conclusion, L-NAME-induced preeclampsia decreases maternal serum hepcidin. Treatment with salicylates modulates iron and zinc status in preeclamptic rats, with specific effects on hepcidin levels.
对于有先兆子痫高风险的女性，推荐使用低剂量阿司匹林进行预防。有人认为膳食水杨酸盐可能有类似作用。尽管水杨酸盐具有已知的抗炎特性，但其对先兆子痫中微量元素的影响仍不清楚。本研究调查了膳食水杨酸盐和阿司匹林对NG-硝基-L-精氨酸甲酯（L-NAME）诱导的先兆子痫大鼠铁、锌和铜状态的影响。该研究涉及将怀孕的Sprague Dawley大鼠分为六组：对照组（CH）、先兆子痫大鼠组（CP）、低剂量膳食水杨酸盐的先兆子痫大鼠组（LSP）、高剂量膳食水杨酸盐的先兆子痫大鼠组、低剂量阿司匹林的先兆子痫大鼠组（LAP）和高剂量阿司匹林的先兆子痫大鼠组。使用火焰原子吸收光谱法测量饮食、肝脏、肾脏、心脏、脾脏、胰腺、股骨、大脑和毛发中的微量元素含量。使用HPLC和UHPLC-MS/MS系统分析饮食、血清和尿液中的水杨酸盐浓度。给予L-NAME导致各组血压升高，只有LAP组的血压水平与CH组相当。先兆子痫显著降低血清铁调素水平，而水杨酸盐消除了这种作用。给予水杨酸盐显著降低毛发中的铁水平，并增加母体大脑中的锌浓度。膳食水杨酸盐显著增加胎盘中的锌水平。总之，L-NAME诱导的先兆子痫降低母体血清铁调素。水杨酸盐治疗可调节先兆子痫大鼠的铁和锌状态，对铁调素水平有特定影响。
