鱿鱼原代细胞的分离、培养及转染方案。

Protocol for the isolation, culture, and transfection of squid primary cells.

作者信息

Kim Yoonji, Tanner Hailey M, Rosenthal Joshua J C, Brangwynne Clifford P

机构信息

Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA; Omenn-Darling Bioengineering Institute, Princeton University, Princeton, NJ 08544, USA.

Department of Chemical and Biological Engineering, Princeton University, Princeton, NJ 08544, USA.

出版信息

STAR Protoc. 2025 Aug 5;6(3):103994. doi: 10.1016/j.xpro.2025.103994.

DOI:10.1016/j.xpro.2025.103994

PMID:40773350

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12347879/

Abstract

We present a protocol for the isolation, culture, and transfection of squid primary cells from various tissues and ages by including steps for squid dissection, cell isolation, maintenance in culture, and expression of exogenous genes. We also highlight life-stage tractability in Euprymna berryi squids for future aging studies. This protocol enables molecular- and cellular-level investigations into processes specific to squids, including their complex behaviors, rapid color change mechanisms, and RNA editing capabilities, with broader implications in basic cell physiology.

摘要

我们提出了一种从不同组织和年龄的鱿鱼中分离、培养和转染原代细胞的方案，其中包括鱿鱼解剖、细胞分离、培养维持以及外源基因表达等步骤。我们还强调了柏氏艾氏鱿鱼在生命阶段方面的易处理性，以便未来进行衰老研究。该方案能够在分子和细胞水平上对鱿鱼特有的过程进行研究，包括它们复杂的行为、快速的颜色变化机制和RNA编辑能力，对基础细胞生理学具有更广泛的意义。