天然细胞膜纳米颗粒系统的研究进展

Advances in native cell membrane nanoparticles system.

作者信息

Qiu Weihua, Guo Youzhong

机构信息

Department of Medicinal Chemistry, Virginia Commonwealth University, Richmond, VA 23298-0540, USA; Center for Drug Discovery, Virginia Commonwealth University, Richmond, VA 23298-0133, USA.

出版信息

Curr Opin Struct Biol. 2025 Aug 6;94:103130. doi: 10.1016/j.sbi.2025.103130.

DOI:10.1016/j.sbi.2025.103130

PMID:40774150

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12374768/

Abstract

The native cell membrane nanoparticles (NCMN) system utilizes membrane-active polymers specifically designed and optimized to extract and stabilize membrane proteins in the form of NCMN particlesfor biochemical and biophysical characterization. The NCMN system is a genuine and advanced detergent-free approach inspired by the membrane activity of the styrene-maleic acid copolymers (SMA), distinguishing it from the nanodisc technology, Salipro technology, and Peptidisc technology. This review introduces the current advancements in the NCMN system, including the development of NCMN polymers, the application of the NCMN system for single-particle cryo-EM analysis, and the functional characterization of membrane proteins.

摘要

天然细胞膜纳米颗粒（NCMN）系统利用经过专门设计和优化的膜活性聚合物，以NCMN颗粒的形式提取和稳定膜蛋白，用于生化和生物物理表征。NCMN系统是一种受苯乙烯-马来酸共聚物（SMA）膜活性启发的真正先进的无去污剂方法，使其有别于纳米盘技术、Salipro技术和肽盘技术。本综述介绍了NCMN系统的当前进展，包括NCMN聚合物的开发、NCMN系统在单颗粒冷冻电镜分析中的应用以及膜蛋白的功能表征。