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优化赤霉素浓度和处理时间以有效打破马铃薯休眠并促进其发芽

Optimizing gibberellic acid concentration and exposure time for effective dormancy breaking and sprouting enhancement in potato.

作者信息

Qayyum Muhammad Mazhar, Shahzad Umbreen, Jahan Muhammad Shah, El-Beltagi Hossam S, Ghuffar Salman, Qasim Muhammad, Mehmood Nasir, Anwaar Sadaf, Qureshi Huma, Anwar Tauseef, Rebouh Nazih Y, Shah Mohd Asif, Muminov Muydin M

机构信息

Department of Horticulture, Kohsar University, Murree, 47200, Pakistan.

Department of Horticulture, University of Layyah, Layyah, 31200, Pakistan.

出版信息

Sci Rep. 2025 Aug 7;15(1):28966. doi: 10.1038/s41598-025-13219-5.

Abstract

Tuber dormancy in Solanum tuberosum L. (potato) significantly limits early growth and yield potential, highlighting the need for effective dormancy-breaking strategies. Gibberellic acid (GA₃) is widely used to stimulate sprouting, but its optimal concentration and exposure time require further evaluation. This study, conducted in 2023 at the Department of Horticulture, University of Haripur, employed a Randomized Complete Block Design (RCBD) to investigate the effects of four GA₃ concentrations (0, 50, 100, and 150 ppm) and four dipping durations (6, 12, 18, and 24 h), totaling 20 treatment combinations. Key parameters measured included sprouting percentage, time to sprout, number of sprouts per tuber, sprout length and diameter, fresh and dry sprout weight, and relative water content. Data were analyzed using one-way ANOVA at P < 0.05. The most effective treatment-150 ppm GA₃ with a 24-hour dipping duration-achieved a 98.33% sprouting rate and the shortest sprouting time (20.45 days). This combination also resulted in the highest number of sprouts (5.63), longest sprout length (10.23 cm), maximum sprout diameter (5.7 mm), greatest fresh (1.18 g) and dry weights (0.31 g), and highest relative water content (83.31%). These findings suggest that high-concentration GA₃ treatments with extended exposure durations effectively break dormancy and enhance sprouting vigor. Future research should explore the biochemical pathways involved and evaluate economic feasibility and field performance under varied agro-climatic conditions for large-scale application.

摘要

马铃薯(Solanum tuberosum L.)的块茎休眠显著限制了其早期生长和产量潜力,这凸显了有效打破休眠策略的必要性。赤霉素(GA₃)被广泛用于刺激发芽,但其最佳浓度和处理时间仍需进一步评估。本研究于2023年在哈里普尔大学园艺系进行,采用随机完全区组设计(RCBD),研究了四种GA₃浓度(0、50、100和150 ppm)和四种浸泡时间(6、12、18和24小时)的影响,共20种处理组合。测量的关键参数包括发芽率、发芽时间、每个块茎的发芽数、芽长和直径、鲜芽和干芽重量以及相对含水量。数据采用P < 0.05的单因素方差分析。最有效的处理方法是150 ppm GA₃浸泡24小时,发芽率达到98.33%,发芽时间最短(20.45天)。这种组合还产生了最多的芽数(5.63个)、最长的芽长(10.23厘米)、最大的芽直径(5.7毫米)、最大的鲜重(1.18克)和干重(0.31克)以及最高的相对含水量(83.31%)。这些结果表明,高浓度GA₃处理并延长处理时间能有效打破休眠并增强发芽活力。未来的研究应探索其中涉及的生化途径,并评估在不同农业气候条件下大规模应用的经济可行性和田间表现。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ba89/12331952/447f7ab6e41a/41598_2025_13219_Fig1_HTML.jpg

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