Hardonk M J, Harms G, Koudstaal J
Histochemistry. 1985;83(5):473-7. doi: 10.1007/BF00509211.
The in vivo uptake in hepatocytes of intravenously injected colloidal gold granules with a diameter of 17 nm or 79 nm and coated with bovine serum albumin or with polyvinyl-pyrrolidone was studied. Irrespective of coating only the 17 nm granules were taken up in hepatocytes. Perivenous hepatocytes did take up much more gold granules than periportal hepatocytes. The gold granules were found in lysosomes around bile canaliculi. Two hours after injection hepatocytes contained the maximal amount of granules. At least a portion of the granules was discharged into the bile. The observed zonal gradient in the uptake of 17 nm gold granules might be caused by the greater supply of granules to the perivenous hepatocytes as a combined result of the higher porosity of the endothelial lining and the smaller number of Kupffer cells with a low endocytic activity in this zone.
研究了静脉注射直径为17nm或79nm且包被有牛血清白蛋白或聚乙烯吡咯烷酮的胶体金颗粒在肝细胞中的体内摄取情况。无论包被与否，只有17nm的颗粒被肝细胞摄取。肝静脉周围的肝细胞摄取的金颗粒比门静脉周围的肝细胞多得多。在胆小管周围的溶酶体中发现了金颗粒。注射后两小时，肝细胞中颗粒含量达到最大值。至少一部分颗粒被排入胆汁。观察到的17nm金颗粒摄取的区域梯度可能是由于该区域内皮衬里孔隙率较高以及吞噬活性较低的库普弗细胞数量较少，导致更多颗粒供应到肝静脉周围的肝细胞。
