生物组织中金的定位。一种用于光学显微镜和电子显微镜的光化学方法。
Localization of gold in biological tissue. A photochemical method for light and electronmicroscopy.
作者信息
Danscher G
出版信息
Histochemistry. 1981;71(1):81-8. doi: 10.1007/BF00592572.
PMID:6785260
Abstract
A detailed description is given of a method by which gold can be visualized in frozen, paraffin and Epon sections. Histological sections from animals treated with gold compounds are exposed to UV-light from 30 min to several hours. The reduced, metallic gold is then visualized by means of a photographic developer containing silver lactate. Light- and electronmicroscope photographs showing gold in different organs from rats and mice treated with aurothioglucose, aurothiosulfate and aurothiomalate are presented.
摘要
本文详细描述了一种可在冷冻切片、石蜡切片和环氧树脂包埋切片中观察到金的方法。用金化合物处理过的动物的组织学切片被置于紫外线下照射30分钟至数小时。然后，通过含有乳酸银的显影剂来观察还原后的金属金。文中展示了用硫代葡萄糖金、硫代硫酸金和硫代苹果酸金处理过的大鼠和小鼠不同器官中含金情况的光学显微镜和电子显微镜照片。
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