de la Maza Diego Sainz, Jefferson Holly, Brucker Celine I, Paoli Sonia, Amoyel Marc
Department of Cell and Developmental Biology, University College London, London, UK.
bioRxiv. 2025 Jul 24:2025.07.22.666113. doi: 10.1101/2025.07.22.666113.
To ensure success in reproduction, organisms dedicate substantial resources to supporting the germline. In testes, somatic gonadal cells form a barrier that isolates germ cells from circulating nutrients, raising the question of how germ cell metabolism is sustained and how somatic cells ensure sufficient resources are directed to the germline. We use lineage-specific manipulations and metabolite reporters to show that somatic gonadal cells break down circulating sugars to produce and shuttle lactate to the germline, sustaining germ cell survival. We show that somatic cells ensure that carbohydrate metabolism is allocated specifically to germ cell support and that increasing autonomous consumption of carbohydrates in the soma increases germ cell death. Thus, germ cell survival depends on correct metabolic compartmentalisation within gonadal somatic support cells.
为确保繁殖成功，生物体投入大量资源来支持生殖细胞系。在睾丸中，体细胞性腺细胞形成一道屏障，将生殖细胞与循环营养物质隔离开来，这就引发了一个问题：生殖细胞的代谢是如何维持的，以及体细胞如何确保有足够的资源导向生殖细胞系。我们利用谱系特异性操作和代谢物报告基因来表明，体细胞性腺细胞分解循环中的糖类以产生乳酸并将其转运至生殖细胞系，从而维持生殖细胞的存活。我们表明，体细胞确保碳水化合物代谢专门用于支持生殖细胞，并且体细胞中碳水化合物自主消耗量的增加会导致生殖细胞死亡。因此，生殖细胞的存活取决于性腺体细胞支持细胞内正确的代谢区室化。
