Nava Iliana, Fuller Margaret T, Gallicchio Lorenzo
Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA, 94305, USA.
Department of Genetics, Stanford University School of Medicine, Stanford, CA, 94305, USA.
bioRxiv. 2025 Jul 22:2025.07.22.666077. doi: 10.1101/2025.07.22.666077.
Stage specific increased expression of PCF11 and Cbc, the two components of Cleavage Factor complex II, contribute to developmentally regulated 3'UTR shortening due to alternate polyadenylation of nascent RNA molecules in spermatocytes compared to spermatogonia. Here, we show that both Cbc and PCF11 change subnuclear localization during male germ cell differentiation, from homogeneous in the nucleus in spermatogonia and early spermatocytes to concentrated around the nucleolus in later spermatocyte stages.
切割因子复合物II的两个组分PCF11和Cbc在特定阶段的表达增加，这导致与精原细胞相比，精子细胞中新生RNA分子因可变聚腺苷酸化而在发育过程中受到调控的3'UTR缩短。在这里，我们表明，在雄性生殖细胞分化过程中，Cbc和PCF11的亚核定位均发生变化，从精原细胞和早期精子细胞中的均匀分布于细胞核，变为后期精子细胞阶段集中在核仁周围。
