Nava Iliana, Fuller Margaret T, Gallicchio Lorenzo
Department of Developmental Biology, Stanford University, Stanford, California, United States.
Department of Genetics, Stanford University, Stanford, California, United States.
MicroPubl Biol. 2025 Aug 15;2025. doi: 10.17912/micropub.biology.001718. eCollection 2025.
Stage specific increased expression of PCF11 and Cbc, the two components of Cleavage Factor complex II, contribute to developmentally regulated 3'UTR shortening due to alternate polyadenylation of nascent RNA molecules in spermatocytes. Here, we show that both Cbc and PCF11 change subnuclear localization during male germ cell differentiation, from homogeneous in the nucleus in spermatogonia and early spermatocytes to concentrated around the nucleolus in later spermatocyte stages.
切割因子复合物II的两个组分PCF11和Cbc在特定阶段的表达增加，这是由于精母细胞中新生RNA分子的可变聚腺苷酸化导致的，有助于发育调控的3'UTR缩短。在这里，我们表明，在雄性生殖细胞分化过程中，Cbc和PCF11的亚核定位都会发生变化，从精原细胞和早期精母细胞核内的均匀分布，变为后期精母细胞阶段围绕核仁的集中分布。
