流式细胞术能否作为检测抗菌药物耐药性的替代方法？

Can flow cytometry be an alternative method for the detection of antimicrobial resistance?

作者信息

Yuruker Ozel, Güler Emrah, Süer Kaya, Güvenir Meryem

机构信息

University of Kyrenia, Faculty of Medicine, Department of Immunology, Kyrenia, Cyprus.

Cyprus Health and Social Sciences, Faculty of Medicine, Department of Medical Microbiology, Guzelyurt, Cyprus.

出版信息

Afr Health Sci. 2024 Sep;24(3):58-62. doi: 10.4314/ahs.v24i3.8.

DOI:10.4314/ahs.v24i3.8

PMID:40777966

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12327143/

Abstract

BACKGROUND

The inappropriate use of antibiotics may cause antibiotic resistance, cause side effects, and eventually cause an increase in healthcare costs. This study aimed to determine a flow cytometry-based test to detect ESBL and MDR in a short time.

METHODS

The study included 25 isolates and 25 isolates identified by Phoenix TM 100 automated system [Becton Dickinson, USA]. In the flow cytometric method, the percentages of death cells exposed to cephalosporin including ceftazidime [CAZ] or cefotaxime [CTX] and clavulanic acid [CLA] combination, were compared with death cells exposed only to a cephalosporin [CAZ or CTX]. CLA index values [CAZ-CLA and CTX-CLA indices] were obtained for CTX and CAZ. Index values that were higher than 1.5 just for one cephalosporin were accepted as positive.

RESULTS

Cell viability was performed after 1-hour exposure to the drugs. When PI staining was applied to ESBL-treated and MDR-treated bacteria, 65% and 85% had nonviable membranes, indicating the method efficiently identified only cells with damaged membranes.

CONCLUSION

Flow cytometry is a rapid and reliable method for the detection of ESBL and MDR in clinical microbiology laboratories.

摘要

背景

抗生素的不当使用可能导致抗生素耐药性，引发副作用，并最终导致医疗成本增加。本研究旨在确定一种基于流式细胞术的检测方法，以便在短时间内检测超广谱β-内酰胺酶（ESBL）和多重耐药性（MDR）。

方法

本研究纳入了由美国贝克顿·迪金森公司的Phoenix TM 100自动化系统鉴定的25株[具体细菌名称未给出]分离株和25株[具体细菌名称未给出]分离株。在流式细胞术方法中，将暴露于头孢他啶（CAZ）或头孢噻肟（CTX）与克拉维酸（CLA）组合的头孢菌素的死亡细胞百分比，与仅暴露于一种头孢菌素（CAZ或CTX）的死亡细胞进行比较。获得了CTX和CAZ的CLA指数值（CAZ-CLA和CTX-CLA指数）。仅一种头孢菌素的指数值高于1.5被视为阳性。