尿热休克蛋白70与先天性肾和尿路异常的关系：UTILISE研究
The relationship between urine heat shock protein 70 and congenital anomalies of the kidney and urinary tract: UTILISE study.
作者信息
Aksu Bagdagul, Afonso Alberto Caldas, Akil Ipek, Alpay Harika, Atmis Bahriye, Aydog Ozlem, Bakkaloglu Sevcan, Bayazıt Aysun Karabay, Bayram Meral Torun, Bilge Ilmay, Bulut Ipek Kaplan, Cetinkaya Ayse Pinar Goksu, Comak Elif, Demir Belde Kasap, Dincel Nida, Donmez Osman, Durmus Mehmet Akif, Dursun Hasan, Dusunsel Ruhan, Duzova Ali, Ertan Pelin, Gedikbasi Asuman, Goknar Nilufer, Guven Sercin, Hacihamdioglu Duygu, Jankauskiene Augustina, Kalyoncu Mukaddes, Kavukcu Salih, Kenan Bahriye Uzun, Kucuk Nuran, Kural Bahar, Litwin Mieczysław, Montini Giovanni, Morello William, Obrycki Lukasz, Omer Beyhan, Misirli Ozdemir Ebru, Ozkayin Nese, Paripovic Dusan, Pehlivanoglu Cemile, Saygili Seha, Schaefer Franz, Schaefer Susanne, Sonmez Ferah, Tabel Yilmaz, Tas Nesrin, Tasdemir Mehmet, Teixeira Ana, Tekcan Demet, Topaloglu Rezan, Tulpar Sebahat, Turkkan Ozde Nisa, Uysal Berfin, Uysalol Metin, Vitkevic Renata, Yavuz Sevgi, Yel Sibel, Yildirim Tarik, Yildirim Zeynep Yuruk, Yildiz Nurdan, Yuksel Selcuk, Yurtseven Eray, Yilmaz Alev
机构信息
Department of Pediatrics Basic Sciences, Institute of Child Health, Istanbul University, Istanbul, Türkiye.
Division of Pediatric Nephrology, Istanbul Faculty of Medicine, Istanbul University, Istanbul, Türkiye.
出版信息
Front Urol. 2024 Feb 28;3:1281081. doi: 10.3389/fruro.2023.1281081. eCollection 2023.
BACKGROUND
Congenital anomalies of the kidney and urinary tract (CAKUT) are defined as structural malformations of the kidney and/or urinary tract. Heat shock proteins (HSPs) are expressed in the kidney in response to cellular changes, such as thermal, hemodynamic, osmotic, inflammatory, and mechanical stresses. This study aimed to assess uHSP70 levels during acute urinary tract infections (UTI) and non-infection periods in patients with CAKUT, and to evaluate whether uHSP70 is elevated in CAKUT subtypes.
METHODS
Among patients with CAKUT, 89 patients with UTI (CAKUT-A), 111 without UTI (CAKUT-B), and 74 healthy children were included in the study. uHSP70 levels were measured using enzyme-linked immunosorbent assay (ELISA).
RESULTS
uHSP70 level was significantly higher in the CAKUT-A group than in the CAKUT-B and healthy control groups (p < 0.0001). Moreover, the level of uHSP70 was significantly higher in the CAKUT-B group than in the control group (p < 0.0001), but was not different between the CAKUT subtypes (p > 0.05).
CONCLUSION
Urine HSP70 can also be used to predict UTI in patients with CAKUT. Moreover, uHSP70 levels were higher in children with CAKUT during the non-infectious period than in healthy controls. This suggests that children with CAKUT are at risk of chronic non-infectious damage.
背景
先天性肾脏和尿路畸形（CAKUT）被定义为肾脏和/或尿路的结构畸形。热休克蛋白（HSPs）在肾脏中表达以应对细胞变化，如热、血流动力学、渗透压、炎症和机械应激。本研究旨在评估CAKUT患者急性尿路感染（UTI）期间和非感染期的尿HSP70水平，并评估CAKUT各亚型中尿HSP70是否升高。
方法
在CAKUT患者中，89例UTI患者（CAKUT - A组）、111例无UTI患者（CAKUT - B组）和74例健康儿童纳入研究。采用酶联免疫吸附测定（ELISA）测量尿HSP70水平。
结果
CAKUT - A组的尿HSP70水平显著高于CAKUT - B组和健康对照组（p < 0.0001）。此外，CAKUT - B组的尿HSP70水平显著高于对照组（p < 0.0001），但CAKUT各亚型之间无差异（p > 0.05）。
结论
尿HSP70也可用于预测CAKUT患者的UTI。此外，CAKUT患儿在非感染期的尿HSP70水平高于健康对照组。这表明CAKUT患儿存在慢性非感染性损伤的风险。
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